Detailed information of TA system TA156062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2841822..2842462
• Replicon: chromosome
• Accession: NZ_CP053092
• Organism: Mycobacterium tuberculosis strain 4860

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: HGO90_RS13255
• Protein ID: WP_003412970.1
• Coordinates: 2841822..2842241 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: HGO90_RS13260
• Protein ID: WP_003412975.1
• Coordinates: 2842238..2842462 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGO90_RS13225	2837407..2838129	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
HGO90_RS13230	2838646..2838873	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
HGO90_RS13235	2838870..2839271	+	402	WP_003412963.1	PIN domain-containing protein	-
HGO90_RS13240	2839306..2840226	-	921	WP_003412965.1	restriction endonuclease	-
HGO90_RS13245	2840567..2840812	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HGO90_RS13250	2840871..2841821	+	951	WP_003911916.1	Lsr2 family protein	-
HGO90_RS13255	2841822..2842241	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
HGO90_RS13260	2842238..2842462	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
HGO90_RS13265	2842493..2845336	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
HGO90_RS13270	2845408..2845809	-	402	WP_003412981.1	hypothetical protein	-
HGO90_RS13275	2845809..2846279	-	471	WP_003899364.1	transcription antitermination factor NusB	-
HGO90_RS13280	2846282..2846845	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T156062 WP_003412970.1 NZ_CP053092:c2842241-2841822 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T156062 NZ_CP068825:c2716138-2716035 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT156062 WP_003412975.1 NZ_CP053092:c2842462-2842238 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT156062 NZ_CP068825:2715997-2716268 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09