Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mbcAT/RES-TIGR02293
Location 2215272..2216170 Replicon chromosome
Accession NZ_CP053092
Organism Mycobacterium tuberculosis strain 4860

Toxin (Protein)


Gene name mbcT Uniprot ID P64908
Locus tag HGO90_RS10335 Protein ID WP_003410001.1
Coordinates 2215272..2215832 (-) Length 187 a.a.

Antitoxin (Protein)


Gene name mbcA Uniprot ID P64910
Locus tag HGO90_RS10340 Protein ID WP_003410003.1
Coordinates 2215829..2216170 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGO90_RS10305 2210441..2211094 - 654 WP_003409976.1 cutinase Cfp21 -
HGO90_RS10310 2211209..2211439 + 231 WP_003409980.1 DUF167 domain-containing protein -
HGO90_RS10315 2211524..2212435 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
HGO90_RS10320 2212544..2213143 + 600 WP_003409989.1 L-lysine exporter -
HGO90_RS10325 2213559..2213987 + 429 WP_003906713.1 cellulose-binding protein -
HGO90_RS10330 2214213..2214752 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
HGO90_RS10335 2215272..2215832 - 561 WP_003410001.1 RES family NAD+ phosphorylase Toxin
HGO90_RS10340 2215829..2216170 - 342 WP_003410003.1 DUF2384 domain-containing protein Antitoxin
HGO90_RS10345 2216256..2216513 + 258 WP_003410006.1 hypothetical protein -
HGO90_RS10350 2216414..2216749 - 336 WP_003410009.1 dehydrogenase -
HGO90_RS10355 2216838..2217182 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 -
HGO90_RS10360 2217176..2217424 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family -
HGO90_RS10365 2217524..2219839 - 2316 WP_003899120.1 cation transporter ATPase CptG -
HGO90_RS10370 2219836..2220108 - 273 WP_003410017.1 DUF1490 family protein -
HGO90_RS10375 2220161..2220517 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(25-148)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(69-110)


Sequences

Toxin        


Download         Length: 187 a.a.        Molecular weight: 20248.70 Da        Isoelectric Point: 4.5091

>T156054 WP_003410001.1 NZ_CP053092:c2215832-2215272 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Download         Length: 561 bp

>T156054 NZ_CP068825:c1167205-1166987 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12488.33 Da        Isoelectric Point: 4.8359

>AT156054 WP_003410003.1 NZ_CP053092:c2216170-2215829 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Download         Length: 342 bp

>AT156054 NZ_CP068825:c1167605-1167231 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FKG

Antitoxin

Source ID Structure
PDB 6FKG
AlphaFold DB A0A7U4FAE4

References