Detailed information of TA system TA156050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2175817..2176520
• Replicon: chromosome
• Accession: NZ_CP053092
• Organism: Mycobacterium tuberculosis strain 4860

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: HGO90_RS10070
• Protein ID: WP_003409778.1
• Coordinates: 2175817..2176146 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: HGO90_RS10075
• Protein ID: WP_003409780.1
• Coordinates: 2176143..2176520 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGO90_RS10050	2172200..2173270	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
HGO90_RS10055	2173267..2173782	+	516	WP_003409718.1	flavin reductase family protein	-
HGO90_RS10060	2173779..2174840	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
HGO90_RS10065	2174837..2175607	+	771	WP_003409775.1	SDR family oxidoreductase	-
HGO90_RS10070	2175817..2176146	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HGO90_RS10075	2176143..2176520	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
HGO90_RS10080	2176517..2177107	-	591	WP_003409784.1	SEC-C domain-containing protein	-
HGO90_RS10085	2177162..2178526	+	1365	WP_023642649.1	HNH endonuclease	-
HGO90_RS10090	2178681..2179133	-	453	WP_003899095.1	lipoprotein	-
HGO90_RS10095	2179197..2179598	+	402	WP_003409869.1	hypothetical protein	-
HGO90_RS10100	2179591..2179773	-	183	WP_003409870.1	hypothetical protein	-
HGO90_RS10105	2179887..2180237	-	351	WP_003899096.1	hypothetical protein	-
HGO90_RS10110	2180248..2181150	-	903	WP_003899097.1	hypothetical protein	-
HGO90_RS10115	2181171..2181362	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T156050 WP_003409778.1 NZ_CP053092:c2176146-2175817 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T156050 NZ_CP068825:c491199-490942 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT156050 WP_003409780.1 NZ_CP053092:c2176520-2176143 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT156050 NZ_CP068825:c490930-490385 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8