Detailed information of TA system TA156047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1765139..1765752
• Replicon: chromosome
• Accession: NZ_CP053092
• Organism: Mycobacterium tuberculosis strain 4860

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: HGO90_RS08230
• Protein ID: WP_003407786.1
• Coordinates: 1765348..1765752 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: HGO90_RS08225
• Protein ID: WP_009935474.1
• Coordinates: 1765139..1765342 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGO90_RS08195	1760544..1760924	+	381	WP_003407768.1	fumarate reductase subunit C	-
HGO90_RS08200	1760921..1761298	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
HGO90_RS08205	1761366..1761974	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
HGO90_RS08210	1762158..1763306	+	1149	Protein_1612	MMPL family transporter	-
HGO90_RS08215	1763316..1763762	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
HGO90_RS08220	1763797..1765086	+	1290	WP_003407781.1	threonine ammonia-lyase	-
HGO90_RS08225	1765139..1765342	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HGO90_RS08230	1765348..1765752	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
HGO90_RS08235	1765769..1767511	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
HGO90_RS08240	1767504..1769801	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T156047 WP_003407786.1 NZ_CP053092:1765348-1765752 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T156047 NZ_CP068824:c4757560-4757387 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT156047 WP_009935474.1 NZ_CP053092:1765139-1765342 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT156047 NZ_CP068824:c4757884-4757588 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46