Detailed information of TA system TA156041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1240969..1241537
• Replicon: chromosome
• Accession: NZ_CP053092
• Organism: Mycobacterium tuberculosis strain 4860

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: HGO90_RS05895
• Protein ID: WP_003405865.1
• Coordinates: 1241163..1241537 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: HGO90_RS05890
• Protein ID: WP_003405863.1
• Coordinates: 1240969..1241166 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGO90_RS05870	1237010..1237648	-	639	WP_003898730.1	lipid droplet-associated protein	-
HGO90_RS05875	1237738..1238745	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
HGO90_RS05880	1238762..1239745	-	984	WP_003898731.1	hypothetical protein	-
HGO90_RS05885	1239808..1240881	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
HGO90_RS05890	1240969..1241166	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HGO90_RS05895	1241163..1241537	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
HGO90_RS05900	1241740..1242438	+	699	WP_003898733.1	hypothetical protein	-
HGO90_RS05905	1242556..1242741	+	186	WP_003901093.1	hypothetical protein	-
HGO90_RS05910	1242668..1242943	-	276	WP_003405867.1	hypothetical protein	-
HGO90_RS05915	1243186..1243509	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
HGO90_RS05920	1243524..1244234	-	711	WP_003911399.1	hypothetical protein	-
HGO90_RS20655	1244256..1244465	+	210	WP_003911400.1	hypothetical protein	-
HGO90_RS05925	1244417..1245124	-	708	Protein_1164	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T156041 WP_003405865.1 NZ_CP053092:1241163-1241537 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T156041 NZ_CP068824:c4042901-4042503 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT156041 WP_003405863.1 NZ_CP053092:1240969-1241166 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT156041 NZ_CP068824:c4043197-4042904 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07