Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 755342..756134 Replicon chromosome
Accession NZ_CP053092
Organism Mycobacterium tuberculosis strain 4860

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag HGO90_RS03475 Protein ID WP_003403386.1
Coordinates 755697..756134 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag HGO90_RS03470 Protein ID WP_003403381.1
Coordinates 755342..755587 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGO90_RS03440 750362..751867 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
HGO90_RS03445 751948..752958 + 1011 WP_009937374.1 ABC transporter ATP-binding protein -
HGO90_RS03450 753346..753729 - 384 WP_003403365.1 ribonuclease VapC6 -
HGO90_RS03455 753824..753979 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
HGO90_RS03460 754055..754771 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
HGO90_RS03465 755047..755355 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
HGO90_RS03470 755342..755587 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
HGO90_RS03475 755697..756134 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
HGO90_RS03480 756131..756385 - 255 WP_003911263.1 antitoxin VapB7 -
HGO90_RS03485 756499..758862 + 2364 WP_003898547.1 arylsulfatase AtsD -
HGO90_RS03490 758925..759251 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
HGO90_RS03495 759163..759501 + 339 WP_003403405.1 PIN domain-containing protein -
HGO90_RS03500 759498..759671 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T156034 WP_003403386.1 NZ_CP053092:c756134-755697 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T156034 NZ_CP068824:c2702950-2702774 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT156034 WP_003403381.1 NZ_CP053092:c755587-755342 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT156034 NZ_CP068824:c2702728-2702312 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References