Detailed information of TA system TA156027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 696983..697629
• Replicon: chromosome
• Accession: NZ_CP053092
• Organism: Mycobacterium tuberculosis strain 4860

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: HGO90_RS03120
• Protein ID: WP_003403137.1
• Coordinates: 696983..697396 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: HGO90_RS03125
• Protein ID: WP_003403139.1
• Coordinates: 697393..697629 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGO90_RS03100	693066..694616	+	1551	WP_003403119.1	MCE family protein	-
HGO90_RS03105	694668..695060	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
HGO90_RS03110	695057..695314	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
HGO90_RS03115	695497..696732	-	1236	WP_003403128.1	ATP-binding protein	-
HGO90_RS03120	696983..697396	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
HGO90_RS03125	697393..697629	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HGO90_RS03130	697733..698239	-	507	WP_003900973.1	two component system sensor kinase HK1	-
HGO90_RS03135	698353..698883	-	531	WP_003917320.1	two component system sensor kinase HK2	-
HGO90_RS03140	698867..699562	-	696	WP_003918099.1	two-component system response regulator TcrA	-
HGO90_RS03145	699685..699996	+	312	WP_003403164.1	hypothetical protein	-
HGO90_RS03150	700068..701018	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
HGO90_RS03155	701259..701843	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
HGO90_RS03160	701845..702558	+	714	Protein_626	IS607 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T156027 WP_003403137.1 NZ_CP053092:c697396-696983 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T156027 NZ_CP068824:737542-737838 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCTGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT156027 WP_003403139.1 NZ_CP053092:c697629-697393 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT156027 NZ_CP068824:737840-738235 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89