Detailed information of TA system TA156021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 365999..366570
• Replicon: chromosome
• Accession: NZ_CP053092
• Organism: Mycobacterium tuberculosis strain 4860

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: HGO90_RS01595
• Protein ID: WP_003401560.1
• Coordinates: 365999..366301 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: HGO90_RS01600
• Protein ID: WP_003401563.1
• Coordinates: 366349..366570 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGO90_RS01575	361714..362517	-	804	WP_003401540.1	hypothetical protein	-
HGO90_RS01580	362527..363924	-	1398	WP_003401544.1	sulfatase	-
HGO90_RS01585	364103..365632	+	1530	WP_014585493.1	PE family protein	-
HGO90_RS01590	365775..366002	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
HGO90_RS01595	365999..366301	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
HGO90_RS01600	366349..366570	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
HGO90_RS01605	366567..366992	+	426	WP_003401566.1	PIN domain nuclease	-
HGO90_RS01610	367128..367760	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
HGO90_RS01615	367757..368665	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T156021 WP_003401560.1 NZ_CP053092:365999-366301 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T156021 NZ_CP068823:c4719647-4719273 [Escherichia coli]
ATGGCAAAAAGAACTGCGCTTTTTGATACCAATATCCTTATCGATTTGTTCAGCGGACGAATTGAGGCAAAACAGGCTCT
GGAGGCATGGCCCCTACAGAATGCGATTAGTCTGGTCACATGGATGGAGGTGCTGGTTGGCGCAAAAAAATATGATCAAG
AACATCGCACACGGATTGCAATGAGTGCGTTTAACGTTATTGGGATTTCGCAGGATATCGCGGAACGAAGCGTTGCTTTA
AGGCAGGAGTATAGAATGAAACTTCCAGATGCCATTATTCTGGCAACAGCACAGATTTATGGCTTTGAACTGGTAACGCG
AAATACGAGAGATTTTGCGGGAATAGCAGGTGTCGTCACACCCTATGAGCTGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT156021 WP_003401563.1 NZ_CP053092:366349-366570 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT156021 NZ_CP068823:c4719889-4719647 [Escherichia coli]
ATGAGCGCTATGGCAGGTATGGATATGGGCAGAATTTTACTCGATTTATCCGATGAGGTGATTAAGCGACTTGATGATCT
CAAAGTGCAGCGCAATCTTCCTCGCGCAGAGCTATTACGAGAAGCTGTAGATCAATATCTGGAAAATCAATCGAAAACGA
CAATATCCAGTGCTTTTGGTATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGTGAGGAATGG
TAA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48