Detailed information of TA system TA156018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 3753172..3754696
• Replicon: chromosome
• Accession: NZ_CP053086
• Organism: Pelagibacterium halotolerans strain ANSP101

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: HKM20_RS18270
• Protein ID: WP_041528879.1
• Coordinates: 3753785..3754696 (+)
• Length: 304 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: HKM20_RS18265
• Protein ID: WP_083824193.1
• Coordinates: 3753172..3753795 (+)
• Length: 208 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKM20_RS18250	3749283..3750350	+	1068	WP_041528881.1	DUF3800 domain-containing protein	-
HKM20_RS18255	3750428..3751582	-	1155	WP_014132761.1	reverse transcriptase family protein	-
HKM20_RS18260	3751759..3752979	-	1221	WP_014132760.1	hypothetical protein	-
HKM20_RS18265	3753172..3753795	+	624	WP_083824193.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
HKM20_RS18270	3753785..3754696	+	912	WP_041528879.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HKM20_RS18275	3754693..3756123	+	1431	WP_014132757.1	toll/interleukin-1 receptor domain-containing protein	-
HKM20_RS18280	3756113..3757675	+	1563	WP_041528878.1	hypothetical protein	-
HKM20_RS18285	3757744..3759492	-	1749	WP_014132755.1	ATP-dependent helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-257)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (17-56)

Sequences

Toxin

Protein

• Length: 304 a.a.
• Molecular weight: 33508.29 Da
• Isoelectric Point: 4.6558

>T156018 WP_041528879.1 NZ_CP053086:3753785-3754696 [Pelagibacterium halotolerans]
MARKVNNIGASVRARLLARARAENLDFQILLTRYALERLLYRLSISDRRNDFILKGAMLFAVWRDDPFRPTRDLDLLGHG
DPEPAAIAESMRSICSAIVPDDGVIFDIDGIEATPIRDEAEYAGVRVRTSATIAGARVPIQIDVGFGDAITPAALEIEYP
SLLDAPAPMLRAYPPETVIAEKTEAIVSLGVANSRMKDFYDLWMVSQTFAFEGKALTDAVQKTFERRRTPWPEQLPTGLS
DAFASEKDAQWRAFLARDRLAAAPASLIDVADNLRAFLGPVLAREELASWPASGPWMYLEAAE

Nucleotide

• Length: 912 bp

>T156018 NZ_CP068823:c4282359-4282069 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACTGGGATATCGCCTGGTCTATTCCGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAAAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 208 a.a.
• Molecular weight: 23045.87 Da
• Isoelectric Point: 10.3275

>AT156018 WP_083824193.1 NZ_CP053086:3753172-3753795 [Pelagibacterium halotolerans]
MSPPSASQRKTACALLSEKGLVRMREFRDAGVGAETVARLVRDGAVERVARGLYQLSGAMPDPQHNFAEASALVPKGVIC
LTSALQYHDMTLQMPSAVWMAIDRTGWKPKVEYPPIRFVRFGTKAMSEGIKRHQIDGVAVPIFEPAKTIVDCFRYRNKIG
LDIALESLREGLRAKRVTPDQLWKFARSARVWTVMRPYVEAMVADGA

Nucleotide

• Length: 624 bp

>AT156018 NZ_CP068823:c4282591-4282349 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATTACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA