Detailed information of TA system TA156017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 3158670..3159162
• Replicon: chromosome
• Accession: NZ_CP053086
• Organism: Pelagibacterium halotolerans strain ANSP101

Toxin (Protein)

• Gene name: -
• Uniprot ID: G4R8M3
• Locus tag: HKM20_RS15440
• Protein ID: WP_014129459.1
• Coordinates: 3158670..3158933 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: G4R813
• Locus tag: HKM20_RS15445
• Protein ID: WP_014129458.1
• Coordinates: 3158920..3159162 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKM20_RS15420	3153956..3154798	-	843	WP_014129463.1	hypothetical protein	-
HKM20_RS15425	3155087..3155812	-	726	WP_014129462.1	copper homeostasis protein CutC	-
HKM20_RS15430	3155918..3156832	-	915	WP_014129461.1	ROK family protein	-
HKM20_RS15435	3157019..3158530	-	1512	WP_014129460.1	ATP-binding protein	-
HKM20_RS15440	3158670..3158933	+	264	WP_014129459.1	BrnT family toxin	Toxin
HKM20_RS15445	3158920..3159162	+	243	WP_014129458.1	CopG family transcriptional regulator	Antitoxin
HKM20_RS15450	3159216..3159707	+	492	WP_014129457.1	NUDIX hydrolase	-
HKM20_RS15455	3159835..3161337	-	1503	WP_014129456.1	inorganic phosphate transporter	-
HKM20_RS15460	3161551..3162150	+	600	WP_014129455.1	DUF2062 domain-containing protein	-
HKM20_RS15465	3162175..3162591	+	417	WP_014129454.1	VOC family protein	-
HKM20_RS15470	3162594..3163511	-	918	WP_014129453.1	pca operon transcription factor PcaQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9862.16 Da
• Isoelectric Point: 4.8047

>T156017 WP_014129459.1 NZ_CP053086:3158670-3158933 [Pelagibacterium halotolerans]
MIFEYDPAQSAANLEKHGIDFEAAQALWADELALEIPARTTDEPRFLVIGKIGDRHWAAVITYRGAAVRIISVRRARDQE
IAYYESL

Nucleotide

• Length: 264 bp

>T156017 NZ_CP068823:c4182235-4181858 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9150.45 Da
• Isoelectric Point: 8.7682

>AT156017 WP_014129458.1 NZ_CP053086:3158920-3159162 [Pelagibacterium halotolerans]
MKASEFDTKFEAGEDIEGALDFARARRPNLESRRVNVDFPAWVVEGLDRQAKRLGVTRQSLIKMWIAQKLEHPVDGNPKL

Nucleotide

• Length: 243 bp

>AT156017 NZ_CP068823:c4182693-4182325 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAAATCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: G4R8M3

Antitoxin

• AlphaFold DB: G4R813