Detailed information of TA system TA156016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2746472..2747069
• Replicon: chromosome
• Accession: NZ_CP053086
• Organism: Pelagibacterium halotolerans strain ANSP101

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G4RD98
• Locus tag: HKM20_RS13425
• Protein ID: WP_014129873.1
• Coordinates: 2746773..2747069 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HKM20_RS13420
• Protein ID: WP_014129874.1
• Coordinates: 2746472..2746771 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKM20_RS13390	2742551..2742988	+	438	WP_014129880.1	hypothetical protein	-
HKM20_RS13395	2743109..2743789	+	681	WP_041528550.1	ABC transporter ATP-binding protein	-
HKM20_RS13400	2743825..2745114	+	1290	WP_139304996.1	FtsX-like permease family protein	-
HKM20_RS13405	2745143..2745658	+	516	WP_014129877.1	copper chaperone PCu(A)C	-
HKM20_RS13410	2745760..2745966	+	207	WP_014129876.1	hypothetical protein	-
HKM20_RS13415	2745987..2746400	-	414	WP_014129875.1	TlpA family protein disulfide reductase	-
HKM20_RS13420	2746472..2746771	-	300	WP_014129874.1	putative addiction module antidote protein	Antitoxin
HKM20_RS13425	2746773..2747069	-	297	WP_014129873.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HKM20_RS13430	2747212..2748726	+	1515	WP_014129871.1	threonine ammonia-lyase, biosynthetic	-
HKM20_RS13435	2748734..2749723	-	990	WP_014129870.1	CDF family Co(II)/Ni(II) efflux transporter DmeF	-
HKM20_RS13440	2749750..2750022	-	273	WP_041528548.1	metal/formaldehyde-sensitive transcriptional repressor	-
HKM20_RS13445	2750091..2750957	+	867	WP_014129868.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2680007..2747069	67062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10953.67 Da
• Isoelectric Point: 10.5743

>T156016 WP_014129873.1 NZ_CP053086:c2747069-2746773 [Pelagibacterium halotolerans]
MIALIKSAVFDNWLRDLRDRRAMARILARLDRFANGNPGDVKAVGGGISEMRIDYGPGYRVYYMQRGPVVIILLSGGDKS
SQKADIARAREIAAQWKD

Nucleotide

• Length: 297 bp

>T156016 NZ_CP068823:c4122674-4122387 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10696.04 Da
• Isoelectric Point: 4.7914

>AT156016 WP_014129874.1 NZ_CP053086:c2746771-2746472 [Pelagibacterium halotolerans]
MTSKETFSRYDSADYLKSEQDIVAYLEAVIEEGGDDPVYIAQALGVVARAHNMSQLARDTGMSREGLYKALSEDGNPSFA
TIVKVAHALGLRLGFKPAA

Nucleotide

• Length: 300 bp

>AT156016 NZ_CP068823:c4122400-4122098 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCCTCGGTGCGTATTGATTCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: G4RD98