Detailed information of TA system TA156015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1576280..1576842
• Replicon: chromosome
• Accession: NZ_CP053086
• Organism: Pelagibacterium halotolerans strain ANSP101

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G4REA2
• Locus tag: HKM20_RS07720
• Protein ID: WP_014131015.1
• Coordinates: 1576471..1576842 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G4REA3
• Locus tag: HKM20_RS07715
• Protein ID: WP_014131016.1
• Coordinates: 1576280..1576474 (+)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKM20_RS07700	1571631..1572041	+	411	WP_014131019.1	hypothetical protein	-
HKM20_RS07705	1572038..1572787	+	750	WP_014131018.1	hypothetical protein	-
HKM20_RS07710	1572939..1576226	+	3288	WP_139305022.1	hypothetical protein	-
HKM20_RS07715	1576280..1576474	+	195	WP_014131016.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HKM20_RS07720	1576471..1576842	+	372	WP_014131015.1	PIN domain-containing protein	Toxin
HKM20_RS07725	1576839..1577741	-	903	WP_083824018.1	flagellar type III secretion system pore protein FliP	-
HKM20_RS07730	1578387..1578791	+	405	WP_014131012.1	flagellar basal body rod protein FlgB	-
HKM20_RS07735	1578803..1579216	+	414	WP_014131011.1	flagellar basal body rod protein FlgC	-
HKM20_RS07740	1579229..1579552	+	324	WP_014131010.1	flagellar hook-basal body complex protein FliE	-
HKM20_RS07745	1579734..1580000	+	267	WP_014131009.1	flagellar biosynthesis protein FliQ	-
HKM20_RS07750	1580007..1580768	+	762	WP_014131008.1	flagellar type III secretion system protein FliR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-110)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13592.79 Da
• Isoelectric Point: 6.0766

>T156015 WP_014131015.1 NZ_CP053086:1576471-1576842 [Pelagibacterium halotolerans]
MILVDTSVWIDHFRAVDEELELLLGANRVLMHPFVLGELALGNLRQRNLILTSLADLPQCVVARDDEVLEFISLHALFGR
GIGYVDAHLLSAAALTPEARLATRDRRLRDRAQSLGLAAALAR

Nucleotide

• Length: 372 bp

>T156015 NZ_CP068823:c4007200-4006886 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTAG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGATAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7099.27 Da
• Isoelectric Point: 10.5887

>AT156015 WP_014131016.1 NZ_CP053086:1576280-1576474 [Pelagibacterium halotolerans]
MRTTIAIDDELVEKAKSLTGLNEKSALVREGLKALIERESARRLALLGGTEPQLKDVPRRRTAI

Nucleotide

• Length: 195 bp

>AT156015 NZ_CP068823:c4007436-4007203 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGAAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: G4REA2

Antitoxin

• AlphaFold DB: G4REA3