Detailed information of TA system TA156008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3011223..3011770
• Replicon: chromosome
• Accession: NZ_CP053084
• Organism: Limnobacter sp. SAORIC-580

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HKT17_RS14080
• Protein ID: WP_171100903.1
• Coordinates: 3011223..3011546 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HKT17_RS14085
• Protein ID: WP_171100905.1
• Coordinates: 3011546..3011770 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKT17_RS14055	3006666..3007226	-	561	WP_171100895.1	hypothetical protein	-
HKT17_RS14060	3007438..3008178	+	741	WP_171101548.1	glycosyltransferase	-
HKT17_RS14065	3008175..3009470	-	1296	WP_171100897.1	type II toxin-antitoxin system HipA family toxin	-
HKT17_RS14070	3009460..3009753	-	294	WP_171100899.1	XRE family transcriptional regulator	-
HKT17_RS14075	3009839..3010951	-	1113	WP_171100901.1	glycosyltransferase	-
HKT17_RS14080	3011223..3011546	-	324	WP_171100903.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HKT17_RS14085	3011546..3011770	-	225	WP_171100905.1	antitoxin MazE family protein	Antitoxin
HKT17_RS14090	3011842..3013029	-	1188	WP_205882444.1	lipase	-
HKT17_RS14095	3013022..3013504	-	483	WP_171100908.1	hypothetical protein	-
HKT17_RS14100	3013686..3013946	+	261	WP_171100910.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HKT17_RS14105	3013950..3014354	+	405	WP_171100912.1	type II toxin-antitoxin system VapC family toxin	-
HKT17_RS14110	3014465..3014734	+	270	WP_171100914.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HKT17_RS14115	3014859..3016025	-	1167	WP_171100917.1	nucleotide sugar dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-102)

Antitoxin

• Antitoxin MazE-like (7-72)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11463.44 Da
• Isoelectric Point: 10.0923

>T156008 WP_171100903.1 NZ_CP053084:c3011546-3011223 [Limnobacter sp. SAORIC-580]
MRGDFVTIATQGDFGKPRPALVIQSNLFQEHATLTVLPVTGTLVSAPLLRVTVHPTSENGLQKVSQIMIDKAITVKQEKV
GPAFGRLDATGLIEIERALAVFLGIAK

Nucleotide

• Length: 324 bp

>T156008 NZ_CP068823:c2439188-2438910 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCAGATATCGATATGCCCGGTTACCGATTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTCGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8232.05 Da
• Isoelectric Point: 6.4508

>AT156008 WP_171100905.1 NZ_CP053084:c3011770-3011546 [Limnobacter sp. SAORIC-580]
MANSNVNSRVQKHRNSLRSAGLRPIQIWVPDTRKPGFADECARQSSVVAQSDATDQAIEHLLNESARDIDGWTD

Nucleotide

• Length: 225 bp

>AT156008 NZ_CP068823:c2438910-2438626 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA