Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3811495..3812113 Replicon chromosome
Accession NZ_CP053080
Organism Escherichia coli strain HB37

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HKW69_RS18395 Protein ID WP_001291435.1
Coordinates 3811895..3812113 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HKW69_RS18390 Protein ID WP_000344800.1
Coordinates 3811495..3811869 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKW69_RS18380 3807024..3810173 + 3150 WP_001132481.1 multidrug efflux RND transporter permease subunit -
HKW69_RS18385 3810494..3811191 + 698 Protein_3596 IS1 family transposase -
HKW69_RS18390 3811495..3811869 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HKW69_RS18395 3811895..3812113 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HKW69_RS18400 3812287..3812838 + 552 WP_000102539.1 maltose O-acetyltransferase -
HKW69_RS18405 3812954..3813424 + 471 WP_000136192.1 YlaC family protein -
HKW69_RS18410 3813588..3815138 + 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HKW69_RS18415 3815180..3815533 - 354 WP_000878138.1 DUF1428 family protein -
HKW69_RS18425 3815912..3816223 + 312 WP_000409908.1 MGMT family protein -
HKW69_RS18430 3816254..3816826 - 573 WP_000779830.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T155982 WP_001291435.1 NZ_CP053080:3811895-3812113 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T155982 NZ_CP068822:2585463-2585741 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT155982 WP_000344800.1 NZ_CP053080:3811495-3811869 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT155982 NZ_CP068822:2585741-2586025 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References