Detailed information of TA system TA155970

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2178167..2178998
• Replicon: chromosome
• Accession: NZ_CP053080
• Organism: Escherichia coli strain HB37

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: HKW69_RS10385
• Protein ID: WP_000854814.1
• Coordinates: 2178167..2178541 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HKW69_RS10390
• Protein ID: WP_024174331.1
• Coordinates: 2178630..2178998 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKW69_RS10345	2173563..2174729	+	1167	WP_001297905.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
HKW69_RS10350	2174848..2175321	+	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
HKW69_RS10355	2175519..2176577	+	1059	WP_001200890.1	FUSC family protein	-
HKW69_RS10360	2176749..2177078	+	330	WP_000450409.1	DUF496 family protein	-
HKW69_RS10365	2177179..2177361	-	183	WP_001016348.1	hypothetical protein	-
HKW69_RS10370	2177388..2177561	+	174	WP_032082634.1	transposase domain-containing protein	-
HKW69_RS10375	2177850..2177963	-	114	WP_001161660.1	DUF957 domain-containing protein	-
HKW69_RS10380	2177976..2178170	-	195	WP_000988601.1	hypothetical protein	-
HKW69_RS10385	2178167..2178541	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HKW69_RS10390	2178630..2178998	-	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HKW69_RS10395	2179072..2179293	-	222	WP_000692346.1	DUF987 domain-containing protein	-
HKW69_RS10400	2179362..2179838	-	477	WP_001541535.1	RadC family protein	-
HKW69_RS10405	2179854..2180327	-	474	WP_000855074.1	antirestriction protein	-
HKW69_RS10410	2180590..2181411	-	822	WP_001234571.1	DUF945 domain-containing protein	-
HKW69_RS10415	2181632..2182042	-	411	WP_000846704.1	hypothetical protein	-
HKW69_RS10420	2182058..2182735	-	678	WP_001362823.1	hypothetical protein	-
HKW69_RS10425	2182871..2183941	-	1071	WP_000102631.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T155970 WP_000854814.1 NZ_CP053080:c2178541-2178167 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T155970 NZ_CP068821:c4782319-4782146 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT155970 WP_024174331.1 NZ_CP053080:c2178998-2178630 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT155970 NZ_CP068821:c4782643-4782347 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A829FY50