Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1193424..1194078 Replicon chromosome
Accession NZ_CP053080
Organism Escherichia coli strain HB37

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag HKW69_RS05905 Protein ID WP_000244765.1
Coordinates 1193671..1194078 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HKW69_RS05900 Protein ID WP_000354046.1
Coordinates 1193424..1193690 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKW69_RS05880 1189513..1190946 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
HKW69_RS05885 1190991..1191301 + 311 Protein_1157 ASCH domain-containing protein -
HKW69_RS05890 1191465..1192124 + 660 WP_000250274.1 hemolysin III family protein -
HKW69_RS05895 1192201..1193181 - 981 WP_000886080.1 tRNA-modifying protein YgfZ -
HKW69_RS05900 1193424..1193690 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HKW69_RS05905 1193671..1194078 + 408 WP_000244765.1 protein YgfX Toxin
HKW69_RS05910 1194118..1194639 - 522 WP_001055874.1 flavodoxin FldB -
HKW69_RS05915 1194751..1195647 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HKW69_RS05920 1195672..1196382 + 711 WP_000715229.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HKW69_RS05925 1196388..1198121 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T155967 WP_000244765.1 NZ_CP053080:1193671-1194078 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T155967 NZ_CP068821:c4612240-4611950 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT155967 WP_000354046.1 NZ_CP053080:1193424-1193690 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT155967 NZ_CP068821:c4612472-4612230 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References