Detailed information of TA system TA155965

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 978962..979796
• Replicon: chromosome
• Accession: NZ_CP053080
• Organism: Escherichia coli strain HB37

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A838AQY5
• Locus tag: HKW69_RS04780
• Protein ID: WP_023152077.1
• Coordinates: 978962..979339 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HKW69_RS04785
• Protein ID: WP_065377585.1
• Coordinates: 979428..979796 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKW69_RS04750	974087..975235	-	1149	WP_000905922.1	capsule polysaccharide export inner-membrane protein KpsE	-
HKW69_RS04755	975307..976290	-	984	WP_001331698.1	KpsF/GutQ family sugar-phosphate isomerase	-
HKW69_RS04760	977089..977259	-	171	Protein_935	IS110 family transposase	-
HKW69_RS04765	977662..978171	-	510	WP_050582268.1	DUF4942 domain-containing protein	-
HKW69_RS04770	978268..978465	-	198	WP_000839281.1	DUF957 domain-containing protein	-
HKW69_RS04775	978477..978965	-	489	WP_065377586.1	hypothetical protein	-
HKW69_RS04780	978962..979339	-	378	WP_023152077.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HKW69_RS04785	979428..979796	-	369	WP_065377585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
HKW69_RS04790	979959..980180	-	222	WP_000692305.1	DUF987 domain-containing protein	-
HKW69_RS04795	980243..980719	-	477	WP_001186773.1	RadC family protein	-
HKW69_RS04800	980735..981214	-	480	WP_170171940.1	antirestriction protein	-
HKW69_RS04805	981296..982114	-	819	WP_032250973.1	DUF945 domain-containing protein	-
HKW69_RS04810	982214..982447	-	234	WP_001119729.1	DUF905 family protein	-
HKW69_RS04815	982526..982981	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	977089..977193	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14056.12 Da
• Isoelectric Point: 7.8839

>T155965 WP_023152077.1 NZ_CP053080:c979339-978962 [Escherichia coli]
MKTLPVLPGQVASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRAIGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T155965 NZ_CP068821:c4439755-4439546 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13580.37 Da
• Isoelectric Point: 5.0580

>AT155965 WP_065377585.1 NZ_CP053080:c979796-979428 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT155965 NZ_CP068821:c4439549-4439247 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A838AQY5