Detailed information of TA system TA155961

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 46902..47700
• Replicon: chromosome
• Accession: NZ_CP053080
• Organism: Escherichia coli strain HB37

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HKW69_RS00235
• Protein ID: WP_019841259.1
• Coordinates: 46902..47279 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HKW69_RS00240
• Protein ID: WP_019841258.1
• Coordinates: 47326..47700 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKW69_RS00205	42819..43317	-	499	Protein_40	hypothetical protein	-
HKW69_RS00210	43910..44188	-	279	Protein_41	DUF4942 domain-containing protein	-
HKW69_RS00215	44476..45480	-	1005	WP_001179707.1	IS110-like element ISSfl8 family transposase	-
HKW69_RS00220	45552..46112	-	561	Protein_43	DUF4942 domain-containing protein	-
HKW69_RS00225	46208..46405	-	198	WP_001609487.1	DUF957 domain-containing protein	-
HKW69_RS00230	46417..46905	-	489	WP_001609486.1	hypothetical protein	-
HKW69_RS00235	46902..47279	-	378	WP_019841259.1	TA system toxin CbtA family protein	Toxin
HKW69_RS00240	47326..47700	-	375	WP_019841258.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HKW69_RS00245	47750..48394	-	645	WP_001609482.1	hypothetical protein	-
HKW69_RS00250	48413..48634	-	222	WP_019841257.1	DUF987 domain-containing protein	-
HKW69_RS00255	48703..49179	-	477	WP_001186725.1	RadC family protein	-
HKW69_RS00260	49195..49674	-	480	WP_019841256.1	antirestriction protein	-
HKW69_RS00265	49756..50574	-	819	WP_019841255.1	DUF945 domain-containing protein	-
HKW69_RS00270	50664..50897	-	234	WP_001278290.1	DUF905 family protein	-
HKW69_RS00275	50903..51580	-	678	WP_001097301.1	hypothetical protein	-
HKW69_RS00280	51728..52408	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	44476..125225	80749
	-	flank	IS/Tn	-	-	44476..45480	1004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14151.12 Da
• Isoelectric Point: 7.1881

>T155961 WP_019841259.1 NZ_CP053080:c47279-46902 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKYPEAKQ

Nucleotide

• Length: 378 bp

>T155961 NZ_CP068821:c3338226-3338123 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13683.52 Da
• Isoelectric Point: 6.0568

>AT155961 WP_019841258.1 NZ_CP053080:c47700-47326 [Escherichia coli]
VSDTLPGTTLPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLACEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT155961 NZ_CP068821:3338085-3338356 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT