Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4619325..4619822 Replicon chromosome
Accession NZ_CP053079
Organism Escherichia coli strain K56-43-un

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HK408_RS22405 Protein ID WP_001173343.1
Coordinates 4619325..4619498 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag HK408_RS22410 Protein ID WP_001312331.1
Coordinates 4619526..4619822 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HK408_RS22395 4615995..4617452 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
HK408_RS22400 4617689..4619206 + 1518 WP_000003806.1 lysine--tRNA ligase -
HK408_RS22405 4619325..4619498 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HK408_RS22410 4619526..4619822 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HK408_RS22415 4620050..4620322 - 273 WP_000405647.1 N-acetyltransferase -
HK408_RS22420 4620334..4620564 - 231 WP_000373293.1 (4Fe-4S)-binding protein -
HK408_RS22425 4620745..4622376 + 1632 WP_001216482.1 sensor histidine kinase -
HK408_RS22430 4622373..4623092 + 720 WP_001540474.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T155959 WP_001173343.1 NZ_CP053079:c4619498-4619325 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T155959 NZ_CP068821:2869415-2869591 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT155959 WP_001312331.1 NZ_CP053079:c4619822-4619526 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT155959 NZ_CP068821:2869637-2870053 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References