Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4234970..4235520 Replicon chromosome
Accession NZ_CP053079
Organism Escherichia coli strain K56-43-un

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HK408_RS20455 Protein ID WP_001160966.1
Coordinates 4234970..4235284 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag HK408_RS20460 Protein ID WP_000125568.1
Coordinates 4235287..4235520 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HK408_RS20430 4230645..4231931 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
HK408_RS20435 4231931..4232920 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HK408_RS20440 4232917..4233738 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HK408_RS20445 4233741..4234118 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HK408_RS20450 4234125..4234973 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HK408_RS20455 4234970..4235284 - 315 WP_001160966.1 CcdB family protein Toxin
HK408_RS20460 4235287..4235520 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HK408_RS20465 4235606..4236085 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
HK408_RS20470 4236277..4238139 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
HK408_RS20475 4238132..4238662 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HK408_RS20480 4238770..4240101 - 1332 WP_001183207.1 MFS transporter -
HK408_RS20485 4240160..4240447 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T155958 WP_001160966.1 NZ_CP053079:c4235284-4234970 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T155958 NZ_CP068821:c2754787-2754593 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT155958 WP_000125568.1 NZ_CP053079:c4235520-4235287 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT155958 NZ_CP068821:2754416-2754594 [Escherichia coli]
GAGGACTGAAGTCTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGGCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References