Detailed information of TA system TA155927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2204158..2204687
• Replicon: chromosome
• Accession: NZ_CP053075
• Organism: Staphylococcus aureus strain SA01

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HK402_RS11120
• Protein ID: WP_000621175.1
• Coordinates: 2204158..2204520 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: HK402_RS11125
• Protein ID: WP_000948331.1
• Coordinates: 2204517..2204687 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HK402_RS11095	2199694..2201013	+	1320	WP_001557163.1	ISL3-like element IS1181 family transposase	-
HK402_RS11100	2201136..2201906	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
HK402_RS11105	2201881..2202360	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
HK402_RS11110	2202362..2202688	-	327	WP_001052491.1	anti-sigma factor antagonist	-
HK402_RS11115	2202807..2203808	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
HK402_RS11120	2204158..2204520	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HK402_RS11125	2204517..2204687	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HK402_RS11130	2204772..2205920	-	1149	WP_001281154.1	alanine racemase	-
HK402_RS11135	2205986..2206345	-	360	WP_000581197.1	holo-ACP synthase	-
HK402_RS11140	2206349..2206840	-	492	WP_001205912.1	PH domain-containing protein	-
HK402_RS11145	2206827..2208410	-	1584	WP_001294626.1	PH domain-containing protein	-
HK402_RS11150	2208403..2208882	-	480	WP_001287087.1	hypothetical protein	-
HK402_RS11155	2209090..2209650	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(M)	-	2180024..2205189	25165
	-	flank	IS/Tn	-	-	2199694..2201013	1319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T155927 WP_000621175.1 NZ_CP053075:c2204520-2204158 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T155927 NZ_CP068820:c2596074-2595724 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT155927 WP_000948331.1 NZ_CP053075:c2204687-2204517 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT155927 NZ_CP068820:c2596319-2596068 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1