Detailed information of TA system TA155914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 2269696..2271179
• Replicon: chromosome
• Accession: NZ_CP053073
• Organism: Usitatibacter palustris strain Swamp67

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: DSM104440_RS11110
• Protein ID: WP_171162593.1
• Coordinates: 2269696..2270574 (-)
• Length: 293 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: DSM104440_RS11115
• Protein ID: WP_171162595.1
• Coordinates: 2270571..2271179 (-)
• Length: 203 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSM104440_RS11090	2264914..2265333	-	420	WP_171162586.1	hypothetical protein	-
DSM104440_RS11095	2265432..2265674	+	243	WP_171162588.1	hypothetical protein	-
DSM104440_RS11100	2265671..2266591	+	921	WP_171162590.1	AraC family transcriptional regulator	-
DSM104440_RS11105	2266568..2269699	-	3132	WP_171162591.1	type I restriction endonuclease subunit R	-
DSM104440_RS11110	2269696..2270574	-	879	WP_171162593.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
DSM104440_RS11115	2270571..2271179	-	609	WP_171162595.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
DSM104440_RS11120	2271246..2272076	-	831	WP_171162597.1	hypothetical protein	-
DSM104440_RS11125	2272073..2273365	-	1293	WP_171162600.1	restriction endonuclease subunit S	-
DSM104440_RS11130	2273355..2274920	-	1566	WP_171162602.1	type I restriction-modification system subunit M	-
DSM104440_RS11135	2275058..2276149	-	1092	WP_171162604.1	DUF4238 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-266)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (12-55)

Sequences

Toxin

Protein

• Length: 293 a.a.
• Molecular weight: 32730.37 Da
• Isoelectric Point: 6.0724

>T155914 WP_171162593.1 NZ_CP053073:c2270574-2269696 [Usitatibacter palustris]
MSGERDGLARSVQVRLARHAKEIGVDPNLVLTRYGVERFLYRLSRSPHAERFVLKGALLLLVWLGENLRPTRDADLLGFG
DMADDELLKIFWEVCDFAVEPDAATFDAGSAKVEPIREGDAYGGRRIVLQGRIGTARVSVQVDIGIGDAVTPEPQWLEYP
SLLGFPSPRLRAYPRETVVAEKVHAMVVLGTRNSRKKDYFDVRALLREDELDETLLVNAIAATFARRRTELPEGIPVGLS
DVFSNDPTHQAQWRAFLAKNRLEGPELADVVNEIREHLKPVLERARGQKVTE

Nucleotide

• Length: 879 bp

>T155914 NZ_CP068820:104140-104394 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAATGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 203 a.a.
• Molecular weight: 22966.70 Da
• Isoelectric Point: 10.7749

>AT155914 WP_171162595.1 NZ_CP053073:c2271179-2270571 [Usitatibacter palustris]
MPPRSNRERTLQLARRARGVTPRELTEQGIHRQVLTRLVEQGQIERIARGVYRLPEQPVSENHGLALAAAAVPQGVICLL
SALQYHGIGTQLPSEVWLALDRRAWRPKLAYPPLRIFRYTGEALTAGAQTHRIEGRLVKIYGPAKTVADCFKYRNKIGLD
VAMEALREGWRARRFTMDELDRYAAICRVQKVMRPYLEALSA

Nucleotide

• Length: 609 bp

>AT155914 NZ_CP068820:103892-104143 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TGCTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA