Detailed information of TA system TA155911

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 2703397..2704505
• Replicon: chromosome
• Accession: NZ_CP053070
• Organism: Staphylococcus aureus strain HL20709 isolate HL20709

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: HH313_RS13455
• Protein ID: WP_000233000.1
• Coordinates: 2703397..2704266 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: HH313_RS13460
• Protein ID: WP_000205227.1
• Coordinates: 2704281..2704505 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HH313_RS13420	2698890..2699627	-	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
HH313_RS13425	2699752..2699835	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
HH313_RS13435	2700377..2701171	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
HH313_RS13440	2701264..2701744	-	481	Protein_2604	GNAT family N-acetyltransferase	-
HH313_RS13445	2701741..2702649	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
HH313_RS13450	2702682..2703416	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
HH313_RS13455	2703397..2704266	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
HH313_RS13460	2704281..2704505	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
HH313_RS13465	2704648..2706192	-	1545	WP_002390960.1	recombinase family protein	-
HH313_RS13470	2706212..2706628	-	417	WP_000323438.1	recombinase	-
HH313_RS13475	2706629..2706925	-	297	WP_002360685.1	hypothetical protein	-
HH313_RS13480	2707437..2708114	-	678	Protein_2612	DNA topoisomerase III	-
HH313_RS13485	2708130..2708732	-	603	WP_001062586.1	recombinase family protein	-
HH313_RS13490	2708746..2708916	-	171	WP_000713595.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	erm(B) / aph(3')-III / ant(6)-Ia	-	2693974..2702649	8675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T155911 WP_000233000.1 NZ_CP053070:c2704266-2703397 [Staphylococcus aureus]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T155911 NZ_CP068819:c4689617-4689444 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT155911 WP_000205227.1 NZ_CP053070:c2704505-2704281 [Staphylococcus aureus]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT155911 NZ_CP068819:c4689941-4689645 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: R3JIN1