Detailed information of TA system TA155898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 3351112..3351765
• Replicon: chromosome
• Accession: NZ_CP053068
• Organism: Mycobacterium avium subsp. paratuberculosis strain DSM 44135

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MAP44135_RS15255
• Protein ID: WP_003872880.1
• Coordinates: 3351112..3351573 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: Q742J1
• Locus tag: MAP44135_RS15260
• Protein ID: WP_003872881.1
• Coordinates: 3351577..3351765 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAP44135_RS15240	3347604..3347990	-	387	WP_003872877.1	hypothetical protein	-
MAP44135_RS15245	3348030..3349613	-	1584	WP_003872878.1	DUF4185 domain-containing protein	-
MAP44135_RS15250	3350609..3351043	-	435	WP_003877359.1	hypothetical protein	-
MAP44135_RS15255	3351112..3351573	-	462	WP_003872880.1	SRPBCC family protein	Toxin
MAP44135_RS15260	3351577..3351765	-	189	WP_003872881.1	antitoxin	Antitoxin
MAP44135_RS15265	3351828..3354200	-	2373	WP_003872882.1	HAD-IC family P-type ATPase	-
MAP44135_RS15270	3354197..3355783	-	1587	WP_003872883.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (2-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-51)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16626.19 Da
• Isoelectric Point: 9.5654

>T155898 WP_003872880.1 NZ_CP053068:c3351573-3351112 [Mycobacterium avium subsp. paratuberculosis]
VAKLSGSIDVPLPPEVAWQHASDLSRYKEWLTIHRVWRSTLPDQIDKGTVVESIVEVKGMLNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKVQPKADGSVVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFARSDPSTNGHR

Nucleotide

• Length: 462 bp

>T155898 NZ_CP068819:c2681295-2681119 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6745.42 Da
• Isoelectric Point: 4.4865

>AT155898 WP_003872881.1 NZ_CP053068:c3351765-3351577 [Mycobacterium avium subsp. paratuberculosis]
MGFLDKAKDLLSQNADKVEMAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKATGATETGDQQG

Nucleotide

• Length: 189 bp

>AT155898 NZ_CP068819:c2681073-2680657 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E2WC18