Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pezAT/ParD(antitoxin)
Location 2271279..2272277 Replicon chromosome
Accession NZ_CP053068
Organism Mycobacterium avium subsp. paratuberculosis strain DSM 44135

Toxin (Protein)


Gene name pezT Uniprot ID X7VT18
Locus tag MAP44135_RS10415 Protein ID WP_003876749.1
Coordinates 2271699..2272277 (+) Length 193 a.a.

Antitoxin (Protein)


Gene name pezA Uniprot ID Q73Z26
Locus tag MAP44135_RS10410 Protein ID WP_003878042.1
Coordinates 2271279..2271668 (+) Length 130 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAP44135_RS10390 2266414..2267094 - 681 WP_010949357.1 hypothetical protein -
MAP44135_RS10395 2267174..2268691 + 1518 WP_015632643.1 carboxylesterase/lipase family protein -
MAP44135_RS10400 2268710..2270707 + 1998 WP_003876752.1 phosphotransferase -
MAP44135_RS10405 2270704..2271198 + 495 WP_010949355.1 gluconokinase -
MAP44135_RS10410 2271279..2271668 + 390 WP_003878042.1 hypothetical protein Antitoxin
MAP44135_RS10415 2271699..2272277 + 579 WP_003876749.1 zeta toxin family protein Toxin
MAP44135_RS10420 2272282..2273349 - 1068 WP_010949354.1 DoxX family membrane protein -
MAP44135_RS10425 2273460..2274020 + 561 WP_003878040.1 pyrazinamidase PncA -
MAP44135_RS10430 2274020..2274820 + 801 WP_003876746.1 nuclear transport factor 2 family protein -
MAP44135_RS10435 2274834..2275184 - 351 WP_003878038.1 DoxX family protein -
MAP44135_RS10440 2275504..2276724 + 1221 WP_010948702.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2275525..2276724 1199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(5-136)

Antitoxin

ParD-like antitoxin of type II bacterial toxin-antitoxin system

(6-106)


Sequences

Toxin        


Download         Length: 193 a.a.        Molecular weight: 21034.18 Da        Isoelectric Point: 10.6678

>T155897 WP_003876749.1 NZ_CP053068:2271699-2272277 [Mycobacterium avium subsp. paratuberculosis]
MERLDVVVGPNGAGKSTFIALTLAPLLPGSVVVNADEIARQRWPQDPASHAYDAAKVAADTRAKLIDLGRSFIAETVFSH
PSKLDLLHAARRAGFTVVLHVLLIPEDLAVERVRHRVRAGGHDVPETKIRERHRRLWTPVAEAMTLADLATGYDNSRLRG
PRVVARLSGGLTVGAVDWPAWAPETLRSRWPV

Download         Length: 579 bp

>T155897 NZ_CP068819:2576210-2577532 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAACCGTTATGCCAGACCGTTATCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
TGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTGCCGGACGCAGAAATCATTAAAGCGGGGAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGGTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGCCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 130 a.a.        Molecular weight: 13569.99 Da        Isoelectric Point: 5.6717

>AT155897 WP_003878042.1 NZ_CP053068:2271279-2271668 [Mycobacterium avium subsp. paratuberculosis]
MAEAVDRVTRVASDLMDSAAAEGARQSRSAKQQLDHWARVGRAVSSQHTASRRRVEAALAGRLSTAEITAEEGVVFNAEI
SAAIDESLARTNYGATLAGQGVTTVALNDDGEIVEHRPDGTAVVLAGRR

Download         Length: 390 bp

>AT155897 NZ_CP068819:2575944-2576210 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TGACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2W6B4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U6EQJ5

References