Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 1974933..1975597 Replicon chromosome
Accession NZ_CP053068
Organism Mycobacterium avium subsp. paratuberculosis strain DSM 44135

Toxin (Protein)


Gene name vapC Uniprot ID Q73YC9
Locus tag MAP44135_RS09130 Protein ID WP_003872398.1
Coordinates 1975190..1975597 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q73YC8
Locus tag MAP44135_RS09125 Protein ID WP_003872399.1
Coordinates 1974933..1975193 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAP44135_RS09105 1970350..1971012 + 663 WP_003872403.1 TetR/AcrR family transcriptional regulator -
MAP44135_RS09110 1971208..1972664 + 1457 Protein_1797 serine/threonine protein kinase -
MAP44135_RS09115 1972866..1974134 - 1269 WP_003872401.1 cytochrome P450 -
MAP44135_RS09120 1974300..1974752 + 453 WP_003872400.1 VOC family protein -
MAP44135_RS09125 1974933..1975193 + 261 WP_003872399.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MAP44135_RS09130 1975190..1975597 + 408 WP_003872398.1 type II toxin-antitoxin system VapC family toxin Toxin
MAP44135_RS09135 1975744..1977288 - 1545 WP_010949452.1 serine/threonine protein kinase -
MAP44135_RS09140 1977525..1978046 + 522 WP_003878215.1 carboxymuconolactone decarboxylase family protein -
MAP44135_RS09145 1978043..1978588 + 546 WP_003878214.1 carboxymuconolactone decarboxylase family protein -
MAP44135_RS09150 1978709..1979239 + 531 WP_003878213.1 TetR/AcrR family transcriptional regulator -
MAP44135_RS09155 1979228..1980397 - 1170 WP_003878212.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-125)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-64)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14418.47 Da        Isoelectric Point: 4.5642

>T155896 WP_003872398.1 NZ_CP053068:1975190-1975597 [Mycobacterium avium subsp. paratuberculosis]
MTARPAAGVLDTSVFIASESGRQLDEARIPDEVATTVVTLAELHAGVLAATTSNVRAQRLATLESIADMEMLPVDDDAVR
MWARLRIHLAEAGRRMRANDLWIAAIAASRGLPVVTQDDDFAALDGAANLAIIRV

Download         Length: 408 bp

>T155896 NZ_CP068819:2267023-2267126 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9635.01 Da        Isoelectric Point: 11.7137

>AT155896 WP_003872399.1 NZ_CP053068:1974933-1975193 [Mycobacterium avium subsp. paratuberculosis]
MSEVASRELRNDTAGVLRRVREGEDVTITINGRAVAMLTPLRPQRRRWLSKAELLSRLRRAQADPGLRQDLATLAGDTTE
DLGPIR

Download         Length: 261 bp

>AT155896 NZ_CP068819:c2267164-2266893 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L5MIC8

Antitoxin

Source ID Structure
AlphaFold DB A0A7L5MIC5

References