Detailed information of TA system TA155895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1195116..1195680
• Replicon: chromosome
• Accession: NZ_CP053068
• Organism: Mycobacterium avium subsp. paratuberculosis strain DSM 44135

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7VBD8
• Locus tag: MAP44135_RS05760
• Protein ID: WP_003875391.1
• Coordinates: 1195309..1195680 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7L5MGM2
• Locus tag: MAP44135_RS05755
• Protein ID: WP_003875390.1
• Coordinates: 1195116..1195322 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAP44135_RS05735	1190369..1191367	+	999	WP_003875386.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
MAP44135_RS05740	1191391..1192599	-	1209	WP_010949696.1	hypothetical protein	-
MAP44135_RS05745	1192730..1193803	+	1074	WP_003878533.1	redox-regulated ATPase YchF	-
MAP44135_RS05750	1194003..1194947	+	945	WP_003875389.1	HNH endonuclease	-
MAP44135_RS05755	1195116..1195322	+	207	WP_003875390.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MAP44135_RS05760	1195309..1195680	+	372	WP_003875391.1	type II toxin-antitoxin system VapC family toxin	Toxin
MAP44135_RS05765	1195780..1196220	+	441	WP_010949694.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
MAP44135_RS05770	1196276..1196551	+	276	WP_003878531.1	hypothetical protein	-
MAP44135_RS05775	1196653..1197051	+	399	WP_003875394.1	VOC family protein	-
MAP44135_RS05780	1197569..1197922	+	354	WP_003875396.1	hypothetical protein	-
MAP44135_RS05785	1198079..1198603	+	525	WP_033713819.1	glycoside hydrolase	-
MAP44135_RS05790	1199014..1199338	+	325	Protein_1144	antibiotic biosynthesis monooxygenase	-
MAP44135_RS05795	1199343..1200026	-	684	WP_003875399.1	hypothetical protein	-
MAP44135_RS05800	1200144..1200341	+	198	WP_016706054.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13770.14 Da
• Isoelectric Point: 6.6003

>T155895 WP_003875391.1 NZ_CP053068:1195309-1195680 [Mycobacterium avium subsp. paratuberculosis]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVECHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Nucleotide

• Length: 372 bp

>T155895 NZ_CP068819:c2031680-2031306 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7231.22 Da
• Isoelectric Point: 11.0634

>AT155895 WP_003875390.1 NZ_CP053068:1195116-1195322 [Mycobacterium avium subsp. paratuberculosis]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRAPADDSR

Nucleotide

• Length: 207 bp

>AT155895 NZ_CP068819:c2032137-2031769 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A0E2WB37

Antitoxin

• AlphaFold DB: A0A7L5MGM2