Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 8447..8969 Replicon plasmid pHNCF11W-tetX4
Accession NZ_CP053047
Organism Escherichia fergusonii strain HNCF11W

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag HG538_RS22880 Protein ID WP_000220560.1
Coordinates 8447..8728 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag HG538_RS22885 Protein ID WP_000121743.1
Coordinates 8718..8969 (-) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HG538_RS22835 3456..3947 - 492 WP_000872475.1 transcription termination factor NusG -
HG538_RS22840 4308..5465 - 1158 WP_000538023.1 hypothetical protein -
HG538_RS22845 5468..6013 - 546 WP_038976855.1 hypothetical protein -
HG538_RS22850 6340..6612 - 273 WP_000160399.1 hypothetical protein -
HG538_RS22855 6625..6774 - 150 WP_000003880.1 hypothetical protein -
HG538_RS22860 7061..7390 - 330 WP_000866648.1 hypothetical protein -
HG538_RS22865 7483..7698 - 216 WP_001180116.1 hypothetical protein -
HG538_RS22870 7688..7933 - 246 WP_000356546.1 hypothetical protein -
HG538_RS22875 7978..8301 - 324 WP_001181903.1 hypothetical protein -
HG538_RS22880 8447..8728 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HG538_RS22885 8718..8969 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
HG538_RS22890 10225..11061 + 837 WP_001050931.1 replication initiation protein -
HG538_RS22895 11101..11547 + 447 WP_001074386.1 hypothetical protein -
HG538_RS22900 11684..12037 + 354 WP_000876434.1 hypothetical protein -
HG538_RS22905 12097..13065 - 969 WP_166501310.1 IS5 family transposase -
HG538_RS22910 13067..13360 - 294 WP_015060510.1 hypothetical protein -
HG538_RS22915 13376..13606 - 231 WP_000051064.1 partitioning protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aadA2 / lnu(F) / tet(X4) / floR / tet(A) / blaSHV-12 - 1..57105 57105


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-86)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T155891 WP_000220560.1 NZ_CP053047:c8728-8447 [Escherichia fergusonii]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T155891 NZ_CP068819:1117464-1117799 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT155891 WP_000121743.1 NZ_CP053047:c8969-8718 [Escherichia fergusonii]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT155891 NZ_CP068819:1117216-1117464 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G3CAN5


Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References