Detailed information of TA system TA155890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 46886..47487
• Replicon: plasmid pHNCF11W-130kb
• Accession: NZ_CP053046
• Organism: Escherichia fergusonii strain HNCF11W

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HG538_RS22390
• Protein ID: WP_001216046.1
• Coordinates: 46886..47266 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: HG538_RS22395
• Protein ID: WP_001190712.1
• Coordinates: 47266..47487 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HG538_RS22365	42326..43810	-	1485	WP_000124157.1	hypothetical protein	-
HG538_RS22370	43810..45003	-	1194	WP_171882177.1	terminase	-
HG538_RS22375	45090..45542	-	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
HG538_RS22380	45631..46674	-	1044	WP_000648827.1	DUF968 domain-containing protein	-
HG538_RS22385	46702..46881	-	180	WP_039022005.1	hypothetical protein	-
HG538_RS22390	46886..47266	-	381	WP_001216046.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HG538_RS22395	47266..47487	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HG538_RS22400	47873..48853	-	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
HG538_RS22405	49116..50504	+	1389	WP_001207923.1	nitrate/nitrite transporter NarU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	floR / dfrA14 / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / qnrS1 / tet(A)	-	1..130643	130643
	-	flank	IS/Tn	-	-	47873..48853	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13534.25 Da
• Isoelectric Point: 5.6343

>T155890 WP_001216046.1 NZ_CP053046:c47266-46886 [Escherichia fergusonii]
MRHISPEELIALHDANISRYGGLPGMSDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Nucleotide

• Length: 381 bp

>T155890 NZ_CP068819:967494-967901 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT155890 WP_001190712.1 NZ_CP053046:c47487-47266 [Escherichia fergusonii]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT155890 NZ_CP068819:967247-967513 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGATGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6