Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3622777..3623431 Replicon chromosome
Accession NZ_CP053045
Organism Escherichia fergusonii strain HNCF11W

Toxin (Protein)


Gene name cptA Uniprot ID F0JPE9
Locus tag HG538_RS17500 Protein ID WP_000244774.1
Coordinates 3622777..3623184 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HG538_RS17505 Protein ID WP_000354046.1
Coordinates 3623165..3623431 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HG538_RS17480 3618734..3620467 - 1734 WP_000813234.1 single-stranded-DNA-specific exonuclease RecJ -
HG538_RS17485 3620473..3621183 - 711 WP_046076902.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HG538_RS17490 3621208..3622104 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HG538_RS17495 3622216..3622737 + 522 WP_001055874.1 flavodoxin FldB -
HG538_RS17500 3622777..3623184 - 408 WP_000244774.1 protein YgfX Toxin
HG538_RS17505 3623165..3623431 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HG538_RS17510 3623683..3624663 + 981 WP_000886104.1 tRNA-modifying protein YgfZ -
HG538_RS17515 3624740..3625399 - 660 WP_000250283.1 hemolysin III family protein -
HG538_RS17520 3625563..3625874 - 312 WP_001182962.1 N(4)-acetylcytidine aminohydrolase -
HG538_RS17525 3625919..3627352 + 1434 WP_024256935.1 6-phospho-beta-glucosidase BglA -
HG538_RS17530 3627400..3628293 - 894 WP_000819362.1 transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16078.05 Da        Isoelectric Point: 11.2511

>T155881 WP_000244774.1 NZ_CP053045:c3623184-3622777 [Escherichia fergusonii]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T155881 NZ_CP068818:c4627783-4627433 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT155881 WP_000354046.1 NZ_CP053045:c3623431-3623165 [Escherichia fergusonii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT155881 NZ_CP068818:c4628028-4627777 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L6ZX35

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References