Detailed information of TA system TA155866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 736308..736858
• Replicon: chromosome
• Accession: NZ_CP053045
• Organism: Escherichia fergusonii strain HNCF11W

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HG538_RS03605
• Protein ID: WP_171881973.1
• Coordinates: 736544..736858 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: HG538_RS03600
• Protein ID: WP_000125566.1
• Coordinates: 736308..736541 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HG538_RS03575	731380..731667	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
HG538_RS03580	731726..733057	+	1332	WP_001183185.1	MFS transporter	-
HG538_RS03585	733165..733695	+	531	WP_000600726.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
HG538_RS03590	733688..735550	+	1863	WP_000377100.1	glutathione-regulated potassium-efflux system protein KefC	-
HG538_RS03595	735743..736222	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
HG538_RS03600	736308..736541	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HG538_RS03605	736544..736858	+	315	WP_171881973.1	CcdB family protein	Toxin
HG538_RS03610	736855..737703	-	849	WP_000257179.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
HG538_RS03615	737710..738087	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
HG538_RS03620	738090..738911	-	822	WP_001065375.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
HG538_RS03625	738908..739897	-	990	WP_000241252.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
HG538_RS03630	739897..741183	-	1287	WP_000800490.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11694.65 Da
• Isoelectric Point: 8.0666

>T155866 WP_171881973.1 NZ_CP053045:736544-736858 [Escherichia fergusonii]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIRPPERLNPILLLVDGKEYVLMTHETATVPANTLG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T155866 NZ_CP068818:1973252-1973506 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT155866 WP_000125566.1 NZ_CP053045:736308-736541 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT155866 NZ_CP068818:1973004-1973255 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9