Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 26085..26662 Replicon plasmid pHNCF44W_NDM-1
Accession NZ_CP053043
Organism Proteus terrae subsp. cibarius strain HNCF44W

Toxin (Protein)


Gene name mazF Uniprot ID U5N651
Locus tag HG539_RS18075 Protein ID WP_023159957.1
Coordinates 26330..26662 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U5N340
Locus tag HG539_RS18070 Protein ID WP_023159984.1
Coordinates 26085..26330 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HG539_RS18030 21090..21434 + 345 WP_023159966.1 single-stranded DNA-binding protein -
HG539_RS18035 21427..21849 + 423 WP_159242432.1 hypothetical protein -
HG539_RS18040 21859..22251 + 393 WP_048821774.1 hypothetical protein -
HG539_RS18045 22532..23011 + 480 WP_159242433.1 Hcp family type VI secretion system effector -
HG539_RS18050 23020..23598 + 579 WP_048822182.1 hypothetical protein -
HG539_RS18055 24359..24727 + 369 WP_048821769.1 hypothetical protein -
HG539_RS18060 24825..25052 - 228 WP_048821767.1 hypothetical protein -
HG539_RS18065 25169..25792 - 624 WP_023159958.1 AAA family ATPase -
HG539_RS18070 26085..26330 + 246 WP_023159984.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HG539_RS18075 26330..26662 + 333 WP_023159957.1 endoribonuclease MazF Toxin
HG539_RS18080 26693..27073 + 381 WP_048821763.1 hypothetical protein -
HG539_RS18085 27162..27302 - 141 WP_072022937.1 type I toxin-antitoxin system Hok family toxin -
HG539_RS18090 27622..28080 - 459 WP_048821762.1 hypothetical protein -
HG539_RS18095 28179..28433 - 255 WP_048821760.1 hypothetical protein -
HG539_RS18100 28472..28741 - 270 WP_048821758.1 hypothetical protein -
HG539_RS18105 29035..29988 + 954 WP_048821757.1 site-specific integrase -
HG539_RS18110 30257..31078 + 822 WP_001029679.1 TnsA endonuclease N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-Ib-cr / ARR-3 / qacE / sul1 / blaNDM-1 / aph(3')-Ia / mph(E) / msr(E) / qnrA1 / aadA2 / dfrA12 / floR / sul2 / aph(4)-Ia / aac(3)-IVa / ant(3'')-Ia / dfrA1 / lnu(F) - 1..139490 139490


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12097.88 Da        Isoelectric Point: 7.0813

>T155856 WP_023159957.1 NZ_CP053043:26330-26662 [Proteus terrae subsp. cibarius]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Download         Length: 333 bp

>T155856 NZ_CP068818:c72958-72581 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCATTTGTCCCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATAACATTACCCAGGGTAAGCATCCGGAGGCGCAACAATGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8786.20 Da        Isoelectric Point: 5.0351

>AT155856 WP_023159984.1 NZ_CP053043:26085-26330 [Proteus terrae subsp. cibarius]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLDNLLAGITPENIHEKVDFGARTGKEL
I

Download         Length: 246 bp

>AT155856 NZ_CP068818:c73416-73048 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCAGATGCATACCACCTGGACCAGGCCTTTGCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P1BQ75

Antitoxin

Source ID Structure
AlphaFold DB A0A857E7M9

References