Detailed information of TA system TA155850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 227801..228444
• Replicon: plasmid pP35A
• Accession: NZ_CP053036
• Organism: Klebsiella pneumoniae strain P35

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HKJ75_RS26595
• Protein ID: WP_014386165.1
• Coordinates: 228028..228444 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HKJ75_RS26590
• Protein ID: WP_032408893.1
• Coordinates: 227801..228031 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKJ75_RS26565	222874..223869	-	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-
HKJ75_RS26570	223876..225024	-	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
HKJ75_RS26575	225195..226352	-	1158	WP_032408895.1	acetylornithine deacetylase	-
HKJ75_RS26580	226368..227042	-	675	WP_014386166.1	DUF1028 domain-containing protein	-
HKJ75_RS26585	227047..227481	-	435	WP_000103648.1	RidA family protein	-
HKJ75_RS26590	227801..228031	+	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HKJ75_RS26595	228028..228444	+	417	WP_014386165.1	PIN domain-containing protein	Toxin
HKJ75_RS26600	228767..229735	+	969	WP_074422984.1	IS5 family transposase	-
HKJ75_RS26605	229915..230289	-	375	WP_032408891.1	hypothetical protein	-
HKJ75_RS26610	230345..230671	-	327	WP_004152639.1	hypothetical protein	-
HKJ75_RS26615	230668..231396	-	729	WP_014386162.1	plasmid SOS inhibition protein A	-
HKJ75_RS26620	231393..231824	-	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1	-	1..248660	248660
	-	flank	IS/Tn	-	-	228812..229735	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T155850 WP_014386165.1 NZ_CP053036:228028-228444 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T155850 NZ_CP068817:c4235407-4235093 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT155850 WP_032408893.1 NZ_CP053036:227801-228031 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT155850 NZ_CP068817:c4235643-4235410 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG