Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53573..54309 Replicon plasmid pP35A
Accession NZ_CP053036
Organism Klebsiella pneumoniae strain P35

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HKJ75_RS25655 Protein ID WP_003026803.1
Coordinates 53827..54309 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HKJ75_RS25650 Protein ID WP_003026799.1
Coordinates 53573..53839 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKJ75_RS25605 49635..49997 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
HKJ75_RS25610 50047..50397 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
HKJ75_RS25615 50755..51024 + 270 WP_004152102.1 hypothetical protein -
HKJ75_RS25620 51012..51587 + 576 WP_004152103.1 hypothetical protein -
HKJ75_RS25625 51618..52112 + 495 WP_004152104.1 DNA-binding protein -
HKJ75_RS25630 52156..52524 + 369 WP_004152105.1 hypothetical protein -
HKJ75_RS25635 52558..52761 + 204 WP_004152106.1 hemolysin expression modulator Hha -
HKJ75_RS25640 52810..53067 + 258 WP_004152107.1 hypothetical protein -
HKJ75_RS25645 53143..53397 + 255 WP_004152108.1 hypothetical protein -
HKJ75_RS25650 53573..53839 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HKJ75_RS25655 53827..54309 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HKJ75_RS25660 54521..55867 + 1347 WP_020314316.1 ISNCY family transposase -
HKJ75_RS25665 56368..57622 - 1255 Protein_57 IS3 family transposase -
HKJ75_RS25670 57710..58672 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1 - 1..248660 248660


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(45-149)

Antitoxin

(6-82)


Sequences


Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T155848 WP_003026803.1 NZ_CP053036:53827-54309 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T155848 NZ_CP068817:c3902878-3902501 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCTGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCCCTGGTGCGTACCGACCAGCCGGGATTC
AGCCCCTGTACCCGCTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT155848 WP_003026799.1 NZ_CP053036:53573-53839 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT155848 NZ_CP068817:c3903336-3902968 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACAGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6


Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References