Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5195485..5196110 Replicon chromosome
Accession NZ_CP053035
Organism Klebsiella pneumoniae strain P35

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HKJ75_RS25015 Protein ID WP_002882817.1
Coordinates 5195485..5195868 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HKJ75_RS25020 Protein ID WP_004150355.1
Coordinates 5195868..5196110 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKJ75_RS25000 5192851..5193753 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HKJ75_RS25005 5193750..5194385 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HKJ75_RS25010 5194382..5195311 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HKJ75_RS25015 5195485..5195868 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HKJ75_RS25020 5195868..5196110 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HKJ75_RS25025 5196315..5197232 + 918 WP_004181612.1 alpha/beta hydrolase -
HKJ75_RS25030 5197246..5198187 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HKJ75_RS25035 5198232..5198669 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HKJ75_RS25040 5198666..5199526 - 861 WP_004146232.1 virulence factor BrkB family protein -
HKJ75_RS25045 5199520..5200119 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T155847 WP_002882817.1 NZ_CP053035:c5195868-5195485 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T155847 NZ_CP068817:3712831-3713049 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT155847 WP_004150355.1 NZ_CP053035:c5196110-5195868 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT155847 NZ_CP068817:3712431-3712805 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References