Detailed information of TA system TA155816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3466037..3467258
• Replicon: chromosome
• Accession: NZ_CP053028
• Organism: Pseudomonas aeruginosa PAO1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HKX07_RS15870
• Protein ID: WP_003114949.1
• Coordinates: 3466680..3467258 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HKX07_RS15865
• Protein ID: WP_003088112.1
• Coordinates: 3466037..3466591 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKX07_RS15840	3461605..3462156	+	552	WP_003102727.1	XRE family transcriptional regulator	-
HKX07_RS15845	3462153..3462551	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HKX07_RS15850	3462564..3462887	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
HKX07_RS15855	3463218..3463679	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HKX07_RS15860	3463684..3465879	+	2196	WP_003114948.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HKX07_RS15865	3466037..3466591	+	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
HKX07_RS15870	3466680..3467258	+	579	WP_003114949.1	HD domain-containing protein	Toxin
HKX07_RS15875	3467366..3468553	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HKX07_RS15880	3468543..3470714	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
HKX07_RS15885	3470704..3471981	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21897.75 Da
• Isoelectric Point: 5.3655

>T155816 WP_003114949.1 NZ_CP053028:3466680-3467258 [Pseudomonas aeruginosa PAO1]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAVRRDA

Nucleotide

• Length: 579 bp

>T155816 NZ_CP068816:2248383-2248485 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT155816 WP_003088112.1 NZ_CP053028:3466037-3466591 [Pseudomonas aeruginosa PAO1]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT155816 NZ_CP068816:c2248523-2248255 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT