Detailed information of TA system TA155814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1589590..1591285
• Replicon: chromosome
• Accession: NZ_CP053028
• Organism: Pseudomonas aeruginosa PAO1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: HKX07_RS07395
• Protein ID: WP_003112901.1
• Coordinates: 1589590..1590459 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: HKX07_RS07400
• Protein ID: WP_003112902.1
• Coordinates: 1590452..1591285 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKX07_RS07370	1585598..1586449	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
HKX07_RS07375	1586490..1587497	+	1008	WP_003112897.1	ABC transporter substrate-binding protein	-
HKX07_RS07380	1587494..1588270	+	777	WP_003112898.1	ABC transporter permease	-
HKX07_RS07385	1588276..1589037	+	762	WP_003112899.1	SDR family oxidoreductase	-
HKX07_RS07390	1589051..1589581	+	531	WP_003112900.1	cupin domain-containing protein	-
HKX07_RS07395	1589590..1590459	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
HKX07_RS07400	1590452..1591285	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
HKX07_RS07405	1591272..1592069	+	798	WP_003112903.1	SDR family oxidoreductase	-
HKX07_RS07410	1592062..1593744	+	1683	WP_003112904.1	thiamine pyrophosphate-binding protein	-
HKX07_RS07415	1593755..1594558	+	804	WP_003112905.1	aspartate dehydrogenase	-
HKX07_RS07420	1594570..1596054	+	1485	WP_003112906.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T155814 WP_003112901.1 NZ_CP053028:1589590-1590459 [Pseudomonas aeruginosa PAO1]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T155814 NZ_CP068816:1949518-1949772 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT155814 WP_003112902.1 NZ_CP053028:1590452-1591285 [Pseudomonas aeruginosa PAO1]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT155814 NZ_CP068816:1949270-1949521 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1