Detailed information of TA system TA155806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 1650932..1651570
• Replicon: chromosome
• Accession: NZ_CP053021
• Organism: Sphingobium yanoikuyae strain YC-XJ2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HH800_RS08360
• Protein ID: WP_048938827.1
• Coordinates: 1651169..1651570 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0J9FLQ9
• Locus tag: HH800_RS08355
• Protein ID: WP_010338822.1
• Coordinates: 1650932..1651165 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HH800_RS08320	1646719..1647144	+	426	WP_159365878.1	PRTRC system protein E	-
HH800_RS08325	1647199..1647405	+	207	WP_159365879.1	hypothetical protein	-
HH800_RS08330	1647417..1647644	+	228	WP_169860753.1	hypothetical protein	-
HH800_RS08335	1647748..1648605	+	858	Protein_1637	hypothetical protein	-
HH800_RS08340	1648617..1649447	+	831	WP_159365880.1	PRTRC system protein B	-
HH800_RS08345	1649444..1650103	+	660	WP_159365881.1	PRTRC system protein A	-
HH800_RS08350	1650093..1650896	+	804	WP_159365882.1	PRTRC system ThiF family protein	-
HH800_RS08355	1650932..1651165	+	234	WP_010338822.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HH800_RS08360	1651169..1651570	+	402	WP_048938827.1	type II toxin-antitoxin system VapC family toxin	Toxin
HH800_RS08365	1651762..1653132	+	1371	WP_159365883.1	hypothetical protein	-
HH800_RS08370	1653663..1654748	+	1086	WP_159365884.1	DUF4297 domain-containing protein	-
HH800_RS08375	1654806..1656494	+	1689	WP_169860754.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1612450..1705313	92863

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-123)

Antitoxin

• SpoVT-AbrB domain (10-55)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14437.62 Da
• Isoelectric Point: 4.7363

>T155806 WP_048938827.1 NZ_CP053021:1651169-1651570 [Sphingobium yanoikuyae]
MFLLDTNILSDLIRQPDGMVAHAIADVGEDAVATSIIVAGELRYGAEKRGSPRLKSKIEDLLSLIAVLPLKDDADACYGR
LRADLERRGTPIGANDMLIAAHALAIGATLVTDNIREFERVDGLQLVNWLRTT

Nucleotide

• Length: 402 bp

>T155806 NZ_CP068815:c4677130-4676957 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8809.19 Da
• Isoelectric Point: 4.8723

>AT155806 WP_010338822.1 NZ_CP053021:1650932-1651165 [Sphingobium yanoikuyae]
MNLERHVKLFKNGRSQAVRIPREFELPGEDAIIRKEGSRLIIEPARPRSLLAVLKTWEPLDEEIGPIEDLPAEPVDI

Nucleotide

• Length: 234 bp

>AT155806 NZ_CP068815:c4677454-4677158 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0J9FLQ9