Detailed information of TA system TA155804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 548..1022
• Replicon: plasmid pKPN361-4
• Accession: NZ_CP053020
• Organism: Klebsiella pneumoniae strain KPN361

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: HJ591_RS28530
• Protein ID: WP_004197762.1
• Coordinates: 756..1022 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: HJ591_RS28525
• Protein ID: WP_000879771.1
• Coordinates: 548..772 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJ591_RS28525	548..772	+	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
HJ591_RS28530	756..1022	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HJ591_RS28535	1092..1442	-	351	WP_004197756.1	hypothetical protein	-
HJ591_RS28540	1508..2053	-	546	WP_023317533.1	hypothetical protein	-
HJ591_RS28545	2079..2458	-	380	Protein_4	hypothetical protein	-
HJ591_RS28550	3561..3737	+	177	WP_004197757.1	hypothetical protein	-
HJ591_RS28555	3727..4071	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
HJ591_RS28560	4010..4774	+	765	Protein_7	relaxase/mobilization nuclease domain-containing protein	-
HJ591_RS28565	4749..5258	+	510	WP_032433972.1	MbeB family mobilization protein	-
HJ591_RS28570	5265..5477	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T155804 WP_004197762.1 NZ_CP053020:756-1022 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T155804 NZ_CP068815:4369093-4369434 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT155804 WP_000879771.1 NZ_CP053020:548-772 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT155804 NZ_CP068815:c4369098-4369022 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin

• AlphaFold DB: S1EJF5