Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 93004..93647 Replicon plasmid pKPN361-1
Accession NZ_CP053017
Organism Klebsiella pneumoniae strain KPN361

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HJ591_RS26745 Protein ID WP_001044770.1
Coordinates 93004..93420 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HJ591_RS26750 Protein ID WP_001261282.1
Coordinates 93417..93647 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HJ591_RS26725 89107..89379 - 273 Protein_111 transposase -
HJ591_RS26730 89408..90105 - 698 Protein_112 IS1 family transposase -
HJ591_RS26735 90361..91383 - 1023 WP_000361404.1 helicase UvrD -
HJ591_RS26740 91368..92930 - 1563 WP_004206609.1 AAA family ATPase -
HJ591_RS26745 93004..93420 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HJ591_RS26750 93417..93647 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HJ591_RS26755 93604..94065 + 462 WP_014343465.1 hypothetical protein -
HJ591_RS26760 94226..95170 + 945 WP_011977810.1 hypothetical protein -
HJ591_RS26765 95207..95599 + 393 WP_011977811.1 hypothetical protein -
HJ591_RS26770 95657..96178 + 522 WP_094960442.1 hypothetical protein -
HJ591_RS26775 96224..96427 + 204 WP_011977813.1 hypothetical protein -
HJ591_RS26780 96457..97461 + 1005 WP_011977814.1 hypothetical protein -
HJ591_RS26785 97645..98424 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2 - 1..169824 169824


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T155801 WP_001044770.1 NZ_CP053017:c93420-93004 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T155801 NZ_CP068815:c3953934-3953536 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT155801 WP_001261282.1 NZ_CP053017:c93647-93417 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT155801 NZ_CP068815:c3954230-3953937 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References