Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5267875..5268500 Replicon chromosome
Accession NZ_CP053016
Organism Klebsiella pneumoniae strain KPN361

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HJ591_RS25805 Protein ID WP_002882817.1
Coordinates 5267875..5268258 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HJ591_RS25810 Protein ID WP_004150355.1
Coordinates 5268258..5268500 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HJ591_RS25790 5265241..5266143 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HJ591_RS25795 5266140..5266775 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HJ591_RS25800 5266772..5267701 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HJ591_RS25805 5267875..5268258 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HJ591_RS25810 5268258..5268500 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HJ591_RS25815 5268705..5269622 + 918 WP_002882812.1 alpha/beta hydrolase -
HJ591_RS25820 5269636..5270577 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HJ591_RS25825 5270622..5271059 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HJ591_RS25830 5271056..5271916 - 861 WP_002882807.1 virulence factor BrkB family protein -
HJ591_RS25835 5271910..5272509 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T155796 WP_002882817.1 NZ_CP053016:c5268258-5267875 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T155796 NZ_CP068815:c3171890-3171507 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT155796 WP_004150355.1 NZ_CP053016:c5268500-5268258 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT155796 NZ_CP068815:c3172351-3171971 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCACCGGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References