Detailed information of TA system TA155773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2069443..2069996
• Replicon: chromosome
• Accession: NZ_CP053014
• Organism: Dickeya zeae strain MS_2018

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HJ586_RS09325
• Protein ID: WP_236306641.1
• Coordinates: 2069443..2069757 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6CEY1
• Locus tag: HJ586_RS09330
• Protein ID: WP_012769207.1
• Coordinates: 2069760..2069996 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJ586_RS09305 (HJ586_09320)	2064584..2066446	+	1863	WP_236306636.1	type IV secretion system DNA-binding domain-containing protein	-
HJ586_RS09310 (HJ586_09325)	2066455..2067201	+	747	WP_236306637.1	MobC family replication-relaxation protein	-
HJ586_RS09315 (HJ586_09330)	2067513..2068160	-	648	WP_236306639.1	hypothetical protein	-
HJ586_RS09320 (HJ586_09335)	2068147..2068470	-	324	WP_022632974.1	DUF1778 domain-containing protein	-
HJ586_RS09325 (HJ586_09340)	2069443..2069757	-	315	WP_236306641.1	CcdB family protein	Toxin
HJ586_RS09330 (HJ586_09345)	2069760..2069996	-	237	WP_012769207.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HJ586_RS09335 (HJ586_09350)	2070317..2071261	-	945	WP_236306642.1	zincin-like metallopeptidase domain-containing protein	-
HJ586_RS09340 (HJ586_09355)	2071967..2074276	+	2310	WP_236306643.1	TerB N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	vgrG1 / hcp	2029767..2090823	61056

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11551.48 Da
• Isoelectric Point: 7.9859

>T155773 WP_236306641.1 NZ_CP053014:c2069757-2069443 [Dickeya zeae]
MQFTVYGNTGKSAVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPAGRRPERLVPVVRLTDGKEYAVMTHELASIPVQALG
TVFCDASQYRTQVKAAIDFLIDGF

Nucleotide

• Length: 315 bp

>T155773 NZ_CP068814:3593718-3594104 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9013.03 Da
• Isoelectric Point: 6.4631

>AT155773 WP_012769207.1 NZ_CP053014:c2069996-2069760 [Dickeya zeae]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLSTALDAELRRHEAKKWQEENREAIDALNRFHDENGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT155773 NZ_CP068814:3594094-3594423 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7W2E4H0