Detailed information of TA system TA155772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/dnstrm_HI1420(antitoxin)
• Location: 2009478..2010228
• Replicon: chromosome
• Accession: NZ_CP053014
• Organism: Dickeya zeae strain MS_2018

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: -
• Locus tag: HJ586_RS09060
• Protein ID: WP_016943626.1
• Coordinates: 2009971..2010228 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: -
• Locus tag: HJ586_RS09055
• Protein ID: WP_236306596.1
• Coordinates: 2009478..2009963 (-)
• Length: 162 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJ586_RS09030 (HJ586_09045)	2005412..2007139	+	1728	WP_012885277.1	hydrogenase large subunit	-
HJ586_RS09035 (HJ586_09050)	2007150..2007719	+	570	WP_016943631.1	hydrogenase 4 subunit H	-
HJ586_RS09040 (HJ586_09055)	2007716..2008504	+	789	WP_012885279.1	NADH-quinone oxidoreductase subunit B family protein	-
HJ586_RS09045 (HJ586_09060)	2008504..2008902	+	399	WP_236306859.1	formate hydrogenlyase maturation HycH family protein	-
HJ586_RS09050 (HJ586_09065)	2008895..2009389	+	495	WP_236306595.1	hydrogenase maturation peptidase HycI	-
HJ586_RS09055 (HJ586_09070)	2009478..2009963	-	486	WP_236306596.1	addiction module antitoxin	Antitoxin
HJ586_RS09060 (HJ586_09075)	2009971..2010228	-	258	WP_016943626.1	DUF4160 domain-containing protein	Toxin
HJ586_RS09065 (HJ586_09080)	2010790..2011818	+	1029	WP_023639411.1	ABC transporter substrate-binding protein	-
HJ586_RS09070 (HJ586_09085)	2011945..2012679	+	735	WP_019844837.1	SDR family oxidoreductase	-
HJ586_RS09075 (HJ586_09090)	2012672..2013709	+	1038	WP_110371746.1	phosphotransferase	-
HJ586_RS09080 (HJ586_09095)	2013748..2015061	+	1314	WP_236306597.1	aminotransferase class III-fold pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-73)

Antitoxin

• Uncharacterized protein HI1420 (80-156)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9839.45 Da
• Isoelectric Point: 7.5565

>T155772 WP_016943626.1 NZ_CP053014:c2010228-2009971 [Dickeya zeae]
MPEIDRLAGLIFHLYFYDDERHHLPHVHVCYGNHELIISIRTGTEIEGYLPNTKRKLAIAHIMAHREQLLSMWAQAVIGK
NPGKL

Nucleotide

• Length: 258 bp

>T155772 NZ_CP068814:c3249492-3249109 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 162 a.a.
• Molecular weight: 17921.33 Da
• Isoelectric Point: 6.6467

>AT155772 WP_236306596.1 NZ_CP053014:c2009963-2009478 [Dickeya zeae]
MHKIVDVDVVGDFSLRLTYDDGLIYDVNLYRYASYPVFSHASQFIRFGLLPDGSLEWPDITISSSALREKGTYVGHTLAV
RERDFADIISRAFYDAVIENRPEILQAALKIAVDKHGNSTVAKEAGAKSRTSLYKSLNKNTNVSLATVVSLAHTVLQLEE
K

Nucleotide

• Length: 486 bp

>AT155772 NZ_CP068814:c3249953-3249573 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG