Detailed information of TA system TA155752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/dnstrm_HI1420(antitoxin)
• Location: 1714536..1715286
• Replicon: chromosome
• Accession: NZ_CP053013
• Organism: Dickeya zeae strain MS_2014

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: -
• Locus tag: HJ580_RS07645
• Protein ID: WP_102801463.1
• Coordinates: 1714536..1714793 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: -
• Locus tag: HJ580_RS07650
• Protein ID: WP_102802692.1
• Coordinates: 1714801..1715286 (+)
• Length: 162 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJ580_RS07625 (HJ580_07640)	1709703..1711016	-	1314	WP_102801461.1	aminotransferase class III-fold pyridoxal phosphate-dependent enzyme	-
HJ580_RS07630 (HJ580_07645)	1711055..1712092	-	1038	WP_102801462.1	phosphotransferase	-
HJ580_RS07635 (HJ580_07650)	1712085..1712819	-	735	WP_019844837.1	SDR family oxidoreductase	-
HJ580_RS07640 (HJ580_07655)	1712946..1713974	-	1029	WP_050568680.1	ABC transporter substrate-binding protein	-
HJ580_RS07645 (HJ580_07660)	1714536..1714793	+	258	WP_102801463.1	DUF4160 domain-containing protein	Toxin
HJ580_RS07650 (HJ580_07665)	1714801..1715286	+	486	WP_102802692.1	addiction module antitoxin	Antitoxin
HJ580_RS07655 (HJ580_07670)	1715372..1715866	-	495	WP_102801464.1	hydrogenase maturation peptidase HycI	-
HJ580_RS07660 (HJ580_07675)	1715859..1716257	-	399	WP_102801465.1	formate hydrogenlyase maturation HycH family protein	-
HJ580_RS07665 (HJ580_07680)	1716257..1717045	-	789	WP_012885279.1	NADH-quinone oxidoreductase subunit B family protein	-
HJ580_RS07670 (HJ580_07685)	1717042..1717611	-	570	WP_016943631.1	hydrogenase 4 subunit H	-
HJ580_RS07675 (HJ580_07690)	1717622..1719349	-	1728	WP_019844844.1	hydrogenase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-73)

Antitoxin

• Uncharacterized protein HI1420 (80-156)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9867.50 Da
• Isoelectric Point: 7.5565

>T155752 WP_102801463.1 NZ_CP053013:1714536-1714793 [Dickeya zeae]
MPEIDRLAGLIFHLYFYDDERHHLPHVHVCYGNHELIISIRTGTEIEGYLPNTKRKLAIAHIMVHREQLLSMWAQAVIGK
NPGKL

Nucleotide

• Length: 258 bp

>T155752 NZ_CP068814:c72958-72584 [Escherichia coli]
ATGAAAACATTACCCGATACACTCGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 162 a.a.
• Molecular weight: 17920.34 Da
• Isoelectric Point: 6.9893

>AT155752 WP_102802692.1 NZ_CP053013:1714801-1715286 [Dickeya zeae]
MHKIVDVDVVGDFSLRLTYDDGLIYDVNLYRYASYPVFSHASQFIRFGLLPDGSLEWPDITISSSALREKGTYVGHTLAV
RERNFADIISRAFYDAVIENRPEILQAALKIAVDKHGNSTVAKEAGAKSRTSLYKSLNKNTNVSLATVVSLAHTVLQLEE
K

Nucleotide

• Length: 486 bp

>AT155752 NZ_CP068814:c73416-73048 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGATATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAACGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAATGAAAAATTAA