Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 700725..701403 Replicon chromosome
Accession NZ_CP053013
Organism Dickeya zeae strain MS_2014

Toxin (Protein)


Gene name cptA Uniprot ID D2BSA8
Locus tag HJ580_RS03205 Protein ID WP_012883372.1
Coordinates 700972..701403 (+) Length 144 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A2K9QKF2
Locus tag HJ580_RS03200 Protein ID WP_016943577.1
Coordinates 700725..700991 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HJ580_RS03185 (HJ580_03185) 697887..698495 - 609 WP_019846260.1 HD domain-containing protein -
HJ580_RS03190 (HJ580_03190) 698708..699358 + 651 WP_038913159.1 hemolysin III family protein -
HJ580_RS03195 (HJ580_03195) 699435..700415 - 981 WP_102801018.1 tRNA-modifying protein YgfZ -
HJ580_RS03200 (HJ580_03200) 700725..700991 + 267 WP_016943577.1 FAD assembly factor SdhE Antitoxin
HJ580_RS03205 (HJ580_03205) 700972..701403 + 432 WP_012883372.1 protein YgfX Toxin
HJ580_RS03210 (HJ580_03210) 701492..702010 - 519 WP_016943578.1 flavodoxin FldB -
HJ580_RS03215 (HJ580_03215) 702187..703512 - 1326 WP_102802665.1 MFS transporter -
HJ580_RS03220 (HJ580_03220) 703776..704675 + 900 WP_016943580.1 site-specific tyrosine recombinase XerD -
HJ580_RS03225 (HJ580_03225) 704762..705478 + 717 WP_019846265.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(2-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 144 a.a.        Molecular weight: 16887.54 Da        Isoelectric Point: 11.5023

>T155749 WP_012883372.1 NZ_CP053013:700972-701403 [Dickeya zeae]
VAQWQCDLRVSWRMQLFSLLTHGFLMLMILLAPWPDGYAPLWLGLVTLVVFDFVRSQRSIKSRQGEISLRGDNQLHWQQR
DWQIVRRPWFMRNGVLLSLRAVDGKGQQRLWLASDSMGSEEWRLLRQQLQQPALHGTESSRHH

Download         Length: 432 bp

>T155749 NZ_CP068813:c4635077-4634727 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10404.88 Da        Isoelectric Point: 5.5981

>AT155749 WP_016943577.1 NZ_CP053013:700725-700991 [Dickeya zeae]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDDDKQQFIRLLQCDDPDLFNWLMNHGEPGDPGLKRMVSLIQTRN
KDRGPVAM

Download         Length: 267 bp

>AT155749 NZ_CP068813:c4635322-4635071 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K9QB06

Antitoxin

Source ID Structure
AlphaFold DB A0A2K9QKF2

References