Detailed information of TA system TA155736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1689225..1689778
• Replicon: chromosome
• Accession: NZ_CP053012
• Organism: Dickeya zeae MS1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: J417_RS07505
• Protein ID: WP_019844797.1
• Coordinates: 1689464..1689778 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: J417_RS07500
• Protein ID: WP_019844796.1
• Coordinates: 1689225..1689461 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J417_RS07485 (J417_07510)	1684926..1687241	-	2316	WP_019844794.1	DEAD/DEAH box helicase family protein	-
J417_RS07490	1687273..1687464	-	192	Protein_1459	helix-turn-helix transcriptional regulator	-
J417_RS07495 (J417_07520)	1687959..1688903	+	945	WP_026357921.1	zincin-like metallopeptidase domain-containing protein	-
J417_RS07500 (J417_07525)	1689225..1689461	+	237	WP_019844796.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
J417_RS07505 (J417_07530)	1689464..1689778	+	315	WP_019844797.1	CcdB family protein	Toxin
J417_RS07510 (J417_07535)	1689775..1690026	-	252	WP_019844798.1	hypothetical protein	-
J417_RS07515	1690088..1690612	+	525	WP_236377707.1	hypothetical protein	-
J417_RS07520	1690584..1690955	+	372	WP_236377708.1	SIR2 family protein	-
J417_RS07525	1690948..1691400	+	453	WP_236377709.1	hypothetical protein	-
J417_RS07530 (J417_07545)	1691652..1691913	+	262	Protein_1467	zincin-like metallopeptidase domain-containing protein	-
J417_RS07535 (J417_07550)	1692349..1693125	+	777	WP_019844800.1	protein kinase	-
J417_RS07540 (J417_07555)	1693122..1693859	+	738	WP_019844801.1	protein phosphatase 2C domain-containing protein	-
J417_RS07545 (J417_07560)	1693910..1694059	+	150	Protein_1470	transposase	-
J417_RS07550 (J417_07565)	1694371..1694700	+	330	WP_019844802.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	hcp	1676725..1728376	51651

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11486.40 Da
• Isoelectric Point: 7.9859

>T155736 WP_019844797.1 NZ_CP053012:1689464-1689778 [Dickeya zeae MS1]
MQFTVYGNTGKSAIYPLLLDVTSDIIGQLNRRIVIPLLPVEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRNQVKAAIDFLIDGI

Nucleotide

• Length: 315 bp

>T155736 NZ_CP068813:1230349-1230684 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8997.03 Da
• Isoelectric Point: 6.4631

>AT155736 WP_019844796.1 NZ_CP053012:1689225-1689461 [Dickeya zeae MS1]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLTAALDAELRRHEAKKWQEENREAIDALNRFHDENGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT155736 NZ_CP068813:1230101-1230349 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA