Detailed information of TA system TA155733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1022594..1023120
• Replicon: chromosome
• Accession: NZ_CP053012
• Organism: Dickeya zeae MS1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: J417_RS04640
• Protein ID: WP_019844363.1
• Coordinates: 1022594..1022899 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6C380
• Locus tag: J417_RS04645
• Protein ID: WP_015855076.1
• Coordinates: 1022902..1023120 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J417_RS04620 (J417_04630)	1017946..1019682	+	1737	WP_019844367.1	ThiF family adenylyltransferase	-
J417_RS04625 (J417_04635)	1019699..1020145	+	447	WP_236377695.1	Mov34/MPN/PAD-1 family protein	-
J417_RS04630 (J417_04640)	1020155..1021222	+	1068	WP_019844365.1	SAVED domain-containing protein	-
J417_RS04635 (J417_04645)	1021809..1022351	-	543	WP_236377735.1	restriction endonuclease	-
J417_RS04640 (J417_04650)	1022594..1022899	-	306	WP_019844363.1	type II toxin-antitoxin system toxin CcdB	Toxin
J417_RS04645 (J417_04655)	1022902..1023120	-	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
J417_RS04650 (J417_04660)	1023179..1023922	-	744	WP_019844362.1	MobC family replication-relaxation protein	-
J417_RS04655 (J417_04665)	1024059..1024229	+	171	WP_172640054.1	hypothetical protein	-
J417_RS04660 (J417_04670)	1024819..1025169	-	351	WP_019844360.1	hypothetical protein	-
J417_RS04665 (J417_04675)	1025356..1025550	+	195	WP_019844359.1	YlcI/YnfO family protein	-
J417_RS04670 (J417_04680)	1025657..1026916	-	1260	WP_019844358.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	989317..1023120	33803

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11750.73 Da
• Isoelectric Point: 6.2252

>T155733 WP_019844363.1 NZ_CP053012:c1022899-1022594 [Dickeya zeae MS1]
MQFIVYKYKRASHYKMFVDVQSDIVDTPKRRMVIPLIESHHLSEKVNKTLFPLIRIEGKDYRLMTTELSSVPVEVMGEAI
ADLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T155733 NZ_CP068813:c883282-882905 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8178.13 Da
• Isoelectric Point: 7.0135

>AT155733 WP_015855076.1 NZ_CP053012:c1023120-1022902 [Dickeya zeae MS1]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT155733 NZ_CP068813:c883740-883372 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A250BG46