Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 459596..460338 Replicon chromosome
Accession NZ_CP053012
Organism Dickeya zeae MS1

Toxin (Protein)


Gene name ataT Uniprot ID E0SIC1
Locus tag J417_RS02085 Protein ID WP_013316117.1
Coordinates 459596..460075 (-) Length 160 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A3N0G0N1
Locus tag J417_RS02090 Protein ID WP_019843650.1
Coordinates 460066..460338 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J417_RS02045 (J417_02050) 455382..455582 + 201 WP_026357784.1 AlpA family transcriptional regulator -
J417_RS02050 455579..455860 + 282 Protein_396 ash family protein -
J417_RS02055 455942..456118 + 177 WP_226100054.1 hypothetical protein -
J417_RS02060 456111..456953 + 843 Protein_398 toprim domain-containing protein -
J417_RS02065 457077..458156 + 1080 WP_026357785.1 helicase RepA family protein -
J417_RS02070 (J417_02065) 458455..458646 - 192 WP_050563618.1 helix-turn-helix transcriptional regulator -
J417_RS02075 (J417_02070) 458759..459022 + 264 WP_019843648.1 helix-turn-helix transcriptional regulator -
J417_RS02080 (J417_02075) 459166..459567 - 402 WP_019843649.1 hypothetical protein -
J417_RS02085 (J417_02080) 459596..460075 - 480 WP_013316117.1 GNAT family N-acetyltransferase Toxin
J417_RS02090 (J417_02085) 460066..460338 - 273 WP_019843650.1 DUF1778 domain-containing protein Antitoxin
J417_RS02095 (J417_02090) 460842..462116 + 1275 Protein_405 integrase arm-type DNA-binding domain-containing protein -
J417_RS02100 (J417_02095) 462317..463191 + 875 Protein_406 HNH endonuclease -
J417_RS02105 (J417_02100) 463604..464209 + 606 WP_026357786.1 DUF6037 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 449932..462116 12184


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(28-147)

Antitoxin

Vibrio phage ICP1, Orf50

(10-85)


Sequences

Toxin        


Download         Length: 160 a.a.        Molecular weight: 17564.34 Da        Isoelectric Point: 9.5367

>T155730 WP_013316117.1 NZ_CP053012:c460075-459596 [Dickeya zeae MS1]
VGITAPELLLPQHAVVDFHCSEPSLNEWLKRKALKNQTLGASRTFVVCEAGTQRVVGFYALASGSIQRQVAPGAFRRNMP
DPIPVLVLGRLAVDERYQRMGIGAGLLKDAVLRSRNVAQQVGNKALLVHALSDEAKAFYQYWGFVPSEIQEHTLLLSLW

Download         Length: 480 bp

>T155730 NZ_CP068813:262820-262927 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10388.86 Da        Isoelectric Point: 4.6702

>AT155730 WP_019843650.1 NZ_CP053012:c460338-460066 [Dickeya zeae MS1]
MRTETKEMPINIRAKASQRELIDVAAKLLSKSRTEFILDAACREAEDVLLDQRLFLVNDEQYDAFIQVLESPVTDNPRVN
ALLNRKSPWE

Download         Length: 273 bp

>AT155730 NZ_CP068813:c262771-262708 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3N0G158

Antitoxin

Source ID Structure
AlphaFold DB A0A3N0G0N1

References