155728

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 647483..648134 Replicon chromosome
Accession NZ_CP052936
Organism Lacticaseibacillus paracasei strain CLP-C10

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag HKJ34_RS03115 Protein ID WP_003567661.1
Coordinates 647751..648134 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag HKJ34_RS03110 Protein ID WP_003567665.1
Coordinates 647483..647731 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKJ34_RS03085 (HKJ34_03155) 642553..643941 + 1389 WP_003581096.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
HKJ34_RS03090 (HKJ34_03160) 643972..644115 + 144 WP_016380391.1 hypothetical protein -
HKJ34_RS03095 (HKJ34_03165) 644241..645749 + 1509 WP_003567670.1 DEAD/DEAH box helicase -
HKJ34_RS03100 (HKJ34_03170) 645918..646292 + 375 WP_003567669.1 holo-ACP synthase -
HKJ34_RS03105 (HKJ34_03175) 646279..647415 + 1137 WP_003581082.1 alanine racemase -
HKJ34_RS03110 (HKJ34_03180) 647483..647731 + 249 WP_003567665.1 antitoxin Antitoxin
HKJ34_RS03115 (HKJ34_03185) 647751..648134 + 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HKJ34_RS03120 (HKJ34_03190) 648318..648839 + 522 WP_003567657.1 QueT transporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 612958..658461 45503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T155728 WP_003567661.1 NZ_CP052936:647751-648134 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T155728 NZ_CP068813:206651-206836 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT155728 WP_003567665.1 NZ_CP052936:647483-647731 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT155728 NZ_CP068813:206853-207263 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGTGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References