Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 64872..65473 Replicon plasmid pC21-4
Accession NZ_CP052881
Organism Escherichia coli strain C21

Toxin (Protein)


Gene name doc Uniprot ID F4TND7
Locus tag HJD21_RS24225 Protein ID WP_001216030.1
Coordinates 64872..65252 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag HJD21_RS24230 Protein ID WP_001190712.1
Coordinates 65252..65473 (-) Length 74 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HJD21_RS24200 60312..61796 - 1485 WP_000124150.1 terminase -
HJD21_RS24205 61796..62989 - 1194 WP_000219604.1 terminase -
HJD21_RS24210 63076..63528 - 453 WP_032305380.1 hypothetical protein -
HJD21_RS24215 63617..64660 - 1044 WP_000648833.1 DUF968 domain-containing protein -
HJD21_RS24220 64688..64867 - 180 WP_000113018.1 hypothetical protein -
HJD21_RS24225 64872..65252 - 381 WP_001216030.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HJD21_RS24230 65252..65473 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HJD21_RS24235 65656..67212 + 1557 WP_029401414.1 type I restriction-modification system subunit M -
HJD21_RS24240 67209..68477 + 1269 WP_029401415.1 restriction endonuclease subunit S -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..93854 93854


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-84)

Antitoxin

(1-51)


Sequences


Toxin        


Download         Length: 127 a.a.        Molecular weight: 13601.29 Da        Isoelectric Point: 5.1408

>T155725 WP_001216030.1 NZ_CP052881:c65252-64872 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEDITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T155725 NZ_CP068812:c5178802-5178695 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACTTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT155725 WP_001190712.1 NZ_CP052881:c65473-65252 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT155725 NZ_CP068812:5178850-5178916 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A1M1W8Q3


Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References